CAJ | 학술논문

采用溶液培养方法,研究了不同浓度镉(0、0.05、0.1、0.5和1 m/L)处理7 d对向日葵幼苗生长和生理特性的影响.结果表明:随着镉处理浓度的增加,向日葵幼苗对镉的吸收显著增加.1 mg/L镉浓度处理时,叶、茎和根中镉浓度分别为0.05 mg/L镉处理时的16.3、19.2和581倍;根中积累的镉含最明显高于叶和茎,各浓度根部积累的镉分别为叶和茎的37.8-63倍和29.4-41倍.镉胁迫显著抑制向13葵幼苗生长和叶绿素合成,当镉浓度达1 mg/L时,整株植物生物量和总叶绿素含量分别为对照的55.9%和52.6%.镉胁迫下向13葵幼苗游离脯氨酸和丙二醛(MDA)含量显著增加,1 mg/L镉浓度时,根中含量分别为对照的4和5.8倍.向日葵幼苗可溶性蛋白含量和过氧化物酶(POD)活性变化与镉胁迫浓度呈明显的倒U字型关系,可溶性蛋白含量在0.05 mg/L镉浓度时达到最大值,叶、茎、根中的POD活性分别在0.1、O.1和0.05 mg/L镉浓度时达到最大值.
채용용액배양방법,연구료불동농도력(0、0.05、0.1、0.5화1 m/L)처리7 d대향일규유묘생장화생리특성적영향.결과표명:수착력처리농도적증가,향일규유묘대력적흡수현저증가.1 mg/L력농도처리시,협、경화근중력농도분별위0.05 mg/L력처리시적16.3、19.2화581배;근중적루적력함최명현고우협화경,각농도근부적루적력분별위협화경적37.8-63배화29.4-41배.력협박현저억제향13규유묘생장화협록소합성,당력농도체1 mg/L시,정주식물생물량화총협록소함량분별위대조적55.9%화52.6%.력협박하향13규유묘유리포안산화병이철(MDA)함량현저증가,1 mg/L력농도시,근중함량분별위대조적4화5.8배.향일규유묘가용성단백함량화과양화물매(POD)활성변화여력협박농도정명현적도U자형관계,가용성단백함량재0.05 mg/L력농도시체도최대치,협、경、근중적POD활성분별재0.1、O.1화0.05 mg/L력농도시체도최대치.
A hydroponic culture experiment was conducted to study the effects of different concentrations of cadmium on the growth and physiological characteristics of sunflower seedlings. The seedlings were treated with 0,0. 05,0. 1,O. 5 and 1 mg/L cadmium for periods of 7 days. The results showed that the cadmium absorbed by sunflower seedlings significantly increased with the augmenting of cadmium concentrations. Specifically,the cadmium accumulated in leaves,stems and roots at 1 mg/L cadmium was 16. 3,19.2 and 581 times as much as at 0. 05 mg/L cadmium,respectively. In addition,cadmium concentrations in roots were 37.8～63 and 29.4～41 times more than that in leaves and stems. The growth and chlorophyll contents of sunflower seedlings were sig-nificantly inhibited by cadmium stress. As compared with control,when cadmium concentration reached 1 mg/L,the biomass of whole plants and total chlorophyll contents dropped to 55.9% and 52. 6%. However,cadmium addition increased the malonyldialdehyde (MDA) and free proline contents,and at 1 mg/L cadmium the contents in roots raised 3 and 4. 8 times as against the control. The inverted U-shaped dose-response effects of cadmium on soluble protein contents and the activities of peroxidase (POD) were observed,with maximum effects of soluble protein contents at 0. 05 mg/L cadmium,and at 0. 1,0. 1,0. 05 mg/L cadmium POD in leav-es,stems,roots attained maximum activities,respectively.