CAJ | 학술논문

利用沸水浴法和超声辅助提取法提取鸡腿菇粗多糖,分别得到对应的多糖水浴提取的多糖(WCP)和超声提取的多糖(UCP).采用苯酚硫酸法测得WCP和UCP中的总糖质量分数分别为87.997%和72.937%.用季铵盐沉淀法和Sephadex G200凝胶层析法对WCP和UCP进行分离纯化,得WCP31、UCP31 2个主要组分,用傅立叶红外光谱(FTIR)和原子力显微镜(AFM)对提取多糖的结构进行表征.结果显示:WCP31和UCP31均具有一般多糖类物质的特征吸收峰,WCP31和UCP31的单糖残基均以β吡喃环存在,但超声波可导致部分CO双键断裂形成C-O链接;WCP31呈现大量的近似螺旋聚集体和少量球状聚集体,但是UCP31是较小的分散球状聚集体,说明超声波可断裂多糖的分子链间或链内氢键,导致近似螺旋聚集体的降解.
이용비수욕법화초성보조제취법제취계퇴고조다당,분별득도대응적다당수욕제취적다당(WCP)화초성제취적다당(UCP).채용분분류산법측득WCP화UCP중적총당질량분수분별위87.997%화72.937%.용계안염침정법화Sephadex G200응효층석법대WCP화UCP진행분리순화,득WCP31、UCP31 2개주요조분,용부립협홍외광보(FTIR)화원자력현미경(AFM)대제취다당적결구진행표정.결과현시:WCP31화UCP31균구유일반다당류물질적특정흡수봉,WCP31화UCP31적단당잔기균이β필남배존재,단초성파가도치부분CO쌍건단렬형성C-O련접;WCP31정현대량적근사라선취집체화소량구상취집체,단시UCP31시교소적분산구상취집체,설명초성파가단렬다당적분자련간혹련내경건,도치근사라선취집체적강해.
Polysaccharides were extracted from Coprinus comatus by boiling water process and ultrasonic-assisted process, the obtained polysaccharides were designated as WCP and UCP, respectively.The total sugars of WCP and UCP were 89.997% and 72.937% determined by phenol-sulfuric acid method. The crude polysaccharides were purified by CTAB and Sephadex G-200 in sequence, and the obtained ones were designated as WCP3-1 and UCP3-1. FTIR showed that there existed β-pyranoside in the polysaccharides and AFM showed that WCP3-1 on mica existed in spiral and spherical form, but UCP3-1 existed in spherical form. The ultrasonic collapse of cavitation bubbles generated intense shock waves that damaged the intrachain or interchain hydrogen bonds.