上海交通大学学报（医学版）
上海交通大學學報（醫學版）
상해교통대학학보（의학판）
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2010年
2期
196-199
,共4页
氯胺酮%Morris水迷宫%凋亡
氯胺酮%Morris水迷宮%凋亡
록알동%Morris수미궁%조망
ketamine%Morris water maze%apoptosis
目的 研究氯胺酮麻醉对新生鼠成年后学习记忆功能的影响.方法 12只出生1周SD大鼠,随机分为氯胺酮麻醉(Ket)组和生理盐水对照(Ns)组.Ket组腹腔注射50 mg/kg氯胺酮;Ns组腹腔注射等量生理盐水.8周后利用Morris水迷宫进行定位航行实验和空间搜索实验,获取大鼠对水迷宫的学习和记忆获取能力以及对平台空间位置的记忆保持能力的相关数据,并行组间比较.制备大鼠海马脑片并行免疫组织化学染色,观察凋亡相关因子Bel-2和Bax的表达.结果 与Ns组比较,Ket组大鼠隐匿平台逃避潜伏期明显延长(P<0.05);穿越平台次数减少,活动总路径缩短(P<0.05).海马脑片观察显示,Ket组促凋亡因子Bax表达较Ns组明显;两组间抑凋亡因子Bcl-2表达无明显差异.结论 在新生鼠的脑发育期使用氯胺酮,其成年后的学习记忆功能受损,其机制可能与海马区神经元凋亡有关.
目的 研究氯胺酮痳醉對新生鼠成年後學習記憶功能的影響.方法 12隻齣生1週SD大鼠,隨機分為氯胺酮痳醉(Ket)組和生理鹽水對照(Ns)組.Ket組腹腔註射50 mg/kg氯胺酮;Ns組腹腔註射等量生理鹽水.8週後利用Morris水迷宮進行定位航行實驗和空間搜索實驗,穫取大鼠對水迷宮的學習和記憶穫取能力以及對平檯空間位置的記憶保持能力的相關數據,併行組間比較.製備大鼠海馬腦片併行免疫組織化學染色,觀察凋亡相關因子Bel-2和Bax的錶達.結果 與Ns組比較,Ket組大鼠隱匿平檯逃避潛伏期明顯延長(P<0.05);穿越平檯次數減少,活動總路徑縮短(P<0.05).海馬腦片觀察顯示,Ket組促凋亡因子Bax錶達較Ns組明顯;兩組間抑凋亡因子Bcl-2錶達無明顯差異.結論 在新生鼠的腦髮育期使用氯胺酮,其成年後的學習記憶功能受損,其機製可能與海馬區神經元凋亡有關.
목적 연구록알동마취대신생서성년후학습기억공능적영향.방법 12지출생1주SD대서,수궤분위록알동마취(Ket)조화생리염수대조(Ns)조.Ket조복강주사50 mg/kg록알동;Ns조복강주사등량생리염수.8주후이용Morris수미궁진행정위항행실험화공간수색실험,획취대서대수미궁적학습화기억획취능력이급대평태공간위치적기억보지능력적상관수거,병행조간비교.제비대서해마뇌편병행면역조직화학염색,관찰조망상관인자Bel-2화Bax적표체.결과 여Ns조비교,Ket조대서은닉평태도피잠복기명현연장(P<0.05);천월평태차수감소,활동총로경축단(P<0.05).해마뇌편관찰현시,Ket조촉조망인자Bax표체교Ns조명현;량조간억조망인자Bcl-2표체무명현차이.결론 재신생서적뇌발육기사용록알동,기성년후적학습기억공능수손,기궤제가능여해마구신경원조망유관.
Objective To investigate the effect of ketamine on learning and memory function of adult rats after injection of ketamine at early development stage. Methods Twelve rats born in a week were randomly divided into ketamine anesthesia group(Ket group)and normal saline control group(Ns group).Rats in Ket group were intraperitoneally injected with 50 mg/kg ketamine,and those in Ns group were administrated with same amount of normal saline.After eight weeks,Morris water maze test was adopted to explore the latency in place navigation and spatial probe ability.The sections of hippocampus were obtained,and immunohistochemical staining was employed to detect the expression of Bax and Bcl-2.Results In Morris water maze test,the latency in place navigation of Ket group Was significantly longer than that of Ns group(P<0.05),and the spatial probe ability significantly decreased(P<0.05).It Was revealed by immunohistochemical staining that the expression of Bax in Ket group was significantly higher than that in Ns group,while there was no significant difference in the expression of Bcl-2 between these two groups. Conclusion Application of ketamine in neonatal rats may result in learning and memory impairment in adulthood,and the underlying mechanism may correlate to the apoptosis of neuron in hippocampus region.
