时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
2期
342-344
,共3页
葎草%多糖%分离%抑菌活性
葎草%多糖%分離%抑菌活性
률초%다당%분리%억균활성
Humulus scandens%Polysaccharides%Isolation%Antibacterial activity
目的 优化葎草多糖的超声辅助提取工艺,并研究其抑菌活性.方法 以多糖中的葡萄糖含量为指标,对超声波功率、料液比和超声时间进行L_9(3~4)正交实验,确定超声辅助提取葎草粗多糖的最佳工艺.用DE-52交换柱对葎草粗多糖进行分离,以滤纸片法研究葎草粗多糖和多糖分离组分的抑菌能力.结果 以水为提取溶剂,料液比1:28(g/ml),超声功率120W,超声90 min提取2次,葎草多糖的得率为0.755%.葎草粗多糖的抑菌效果具有一定的剂量依赖性;各多糖组分中HSP-Ⅰ、HSP-Ⅲ、HSP-Ⅳ发挥主要抑菌作用,其中HSP-Ⅰ效果最显著,HSP-Ⅱ抑菌效果不明显,且各组分间表现出协同关系.结论 超声波辅助提取葎草多糖方法可行,得率高.葎草多糖具有抑菌活性.
目的 優化葎草多糖的超聲輔助提取工藝,併研究其抑菌活性.方法 以多糖中的葡萄糖含量為指標,對超聲波功率、料液比和超聲時間進行L_9(3~4)正交實驗,確定超聲輔助提取葎草粗多糖的最佳工藝.用DE-52交換柱對葎草粗多糖進行分離,以濾紙片法研究葎草粗多糖和多糖分離組分的抑菌能力.結果 以水為提取溶劑,料液比1:28(g/ml),超聲功率120W,超聲90 min提取2次,葎草多糖的得率為0.755%.葎草粗多糖的抑菌效果具有一定的劑量依賴性;各多糖組分中HSP-Ⅰ、HSP-Ⅲ、HSP-Ⅳ髮揮主要抑菌作用,其中HSP-Ⅰ效果最顯著,HSP-Ⅱ抑菌效果不明顯,且各組分間錶現齣協同關繫.結論 超聲波輔助提取葎草多糖方法可行,得率高.葎草多糖具有抑菌活性.
목적 우화률초다당적초성보조제취공예,병연구기억균활성.방법 이다당중적포도당함량위지표,대초성파공솔、료액비화초성시간진행L_9(3~4)정교실험,학정초성보조제취률초조다당적최가공예.용DE-52교환주대률초조다당진행분리,이려지편법연구률초조다당화다당분리조분적억균능력.결과 이수위제취용제,료액비1:28(g/ml),초성공솔120W,초성90 min제취2차,률초다당적득솔위0.755%.률초조다당적억균효과구유일정적제량의뢰성;각다당조분중HSP-Ⅰ、HSP-Ⅲ、HSP-Ⅳ발휘주요억균작용,기중HSP-Ⅰ효과최현저,HSP-Ⅱ억균효과불명현,차각조분간표현출협동관계.결론 초성파보조제취률초다당방법가행,득솔고.률초다당구유억균활성.
Objective To optimize the ultrasound-assisted extraction method and to study the antibacterial activity of polysaccharides from Humulus scandens. Methods Taking the content of glucose as observation index, we optimized the extraction process by L_9(34)orthogonal experiment. The Humulus scandens polysaccharides (HSP) was purified into HSP-Ⅰ, HSP-Ⅱ, HSP-Ⅲ and HSP-Ⅳ by ion exchange chromatography with DE-52. The antibacterial activity of each component was tested by means of filter paper.Results The optimal extraction conditions were obtained as follows: water refluxing, a ratio of solid -liquid as 1g:28ml,120W for the intension of ultrasonic power, extracting time 90min and extracting two times. Under aforesaid optimization conditions, the yield of polysaccharides was 0.755%. Antibacterial experiments indicated that the inhibitory effect of crude polysaccharides showed dose-dependent. Among the isolated components, HSP-Ⅰ, HSP-Ⅲ, HSP-Ⅳ played main antibacterial roles and HSP-Ⅱ was ineffective. It was cooperative with different isolated components. Conclusion The yield of Humulus Scandens polysaccharides is high with ultrasound-assisted extraction method. The Humulus scandens polysaccharides has obvious antibacterial action.
