CAJ | 학술논문

目的:提出拟合响应曲面的3水平设计(Box-Behnken设计)优化红旱莲中总黄酮回流提取工艺.方法:以总黄酮得率为评价指标,乙醇浓度、提取温度、提取时间及溶剂量为考察因素,采用Box-Behnken效应面法优化回流提取工艺条件,并进行预测分析.结果:试验表明乙醇浓度和提取温度对红旱莲总黄酮提取量具极显著影响,最优提取工艺为乙醇浓度53.2%,提取温度78.7℃,提取时间2.3 h,液料比13.3 mL/g,二项式拟合复相关系数较高(r=0.984 6),回归模型的提取率预测值与测定值偏差率为-4.01%.结论:Box-Behnken效应面法用于优化红旱莲的提取工艺是可行的,建立的数学模型和实验观察数据相符.
목적:제출의합향응곡면적3수평설계(Box-Behnken설계)우화홍한련중총황동회류제취공예.방법:이총황동득솔위평개지표,을순농도、제취온도、제취시간급용제량위고찰인소,채용Box-Behnken효응면법우화회류제취공예조건,병진행예측분석.결과:시험표명을순농도화제취온도대홍한련총황동제취량구겁현저영향,최우제취공예위을순농도53.2%,제취온도78.7℃,제취시간2.3 h,액료비13.3 mL/g,이항식의합복상관계수교고(r=0.984 6),회귀모형적제취솔예측치여측정치편차솔위-4.01%.결론:Box-Behnken효응면법용우우화홍한련적제취공예시가행적,건립적수학모형화실험관찰수거상부.
AIM:To put forward three-level design(Box-Behnken design)for fitting response surfaces to optimize the extraction technology of total flavonoids from Hypericum ascyron L.METHODS:Four factors,including temperature,reflux time,concentration of ethanol,ratio of solvent to raw material were used to examine the yield of total flavonoids.Prediction was carried out through comparing the observed and predicted values.RESULTS:The results suggested that ethanol concentration and extraction temperature were two statistically significant factors.The optimum conditions of extraction process consisted of the ratio(mL:g)of solvent to material(13.3:1),ethanol concentration(53.2%),extraction temperature(78.7℃)and extraction time(2.3h).Regression coefficient of binomial fitting complex model was as high as 0.984 6.Bias between observed and predicted values was-4.01%.CONCLUSION:Box-Behnken design is success in optimizing the extaction in close agreement with the predicted values of the mathematic model.