西北植物学报
西北植物學報
서북식물학보
ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA
2010年
7期
1344-1350
,共7页
王伟%叶志云%郑景生%黄育民%黄荣裕%王侯聪%江良荣
王偉%葉誌雲%鄭景生%黃育民%黃榮裕%王侯聰%江良榮
왕위%협지운%정경생%황육민%황영유%왕후총%강량영
水稻%粒形%数量性状座位%上位效应%QE互作效应
水稻%粒形%數量性狀座位%上位效應%QE互作效應
수도%립형%수량성상좌위%상위효응%QE호작효응
rice (Oryza sativa L.)%grain shape%QTLs%epistatic effects%QE interaction effects
利用'广陆矮4号'×'佳辐占'水稻重组自交系构建了SSR标记的遗传图谱.联合2007年和2008年获得的两组稻米粒长(GL)、粒宽(GW)、长宽比(L/W)数据应用混合线性模型方法进行QTL定位,并作加性效应、加性×加性上位互作效应以及加性QTL、上位性QTL与环境的互作效应分析.结果显示;(1)在加性效应分析中两个群体共检测到4个控制粒长的QTL,4个控制粒宽的QTL,5个控制长宽比的QTL,贡献率分别为13.81%、15.36%和 16.29%.(2)在上位互作效应分析中两个群体共检测到2对控制粒长的互作QTL,1对控制粒宽的互作QTL,3对控制长宽比的互作QTL,贡献率分别为5.77%、2.59%和7.42%.(3)环境互作检测中,发现共有13个加性QTL和4对QTL的加性×加性上位性与环境产生了互作效应.结果表明,上位性效应和加性效应都影响稻米粒形遗传,QE互作效应也对粒形有着显著的影响.
利用'廣陸矮4號'×'佳輻佔'水稻重組自交繫構建瞭SSR標記的遺傳圖譜.聯閤2007年和2008年穫得的兩組稻米粒長(GL)、粒寬(GW)、長寬比(L/W)數據應用混閤線性模型方法進行QTL定位,併作加性效應、加性×加性上位互作效應以及加性QTL、上位性QTL與環境的互作效應分析.結果顯示;(1)在加性效應分析中兩箇群體共檢測到4箇控製粒長的QTL,4箇控製粒寬的QTL,5箇控製長寬比的QTL,貢獻率分彆為13.81%、15.36%和 16.29%.(2)在上位互作效應分析中兩箇群體共檢測到2對控製粒長的互作QTL,1對控製粒寬的互作QTL,3對控製長寬比的互作QTL,貢獻率分彆為5.77%、2.59%和7.42%.(3)環境互作檢測中,髮現共有13箇加性QTL和4對QTL的加性×加性上位性與環境產生瞭互作效應.結果錶明,上位性效應和加性效應都影響稻米粒形遺傳,QE互作效應也對粒形有著顯著的影響.
이용'엄륙왜4호'×'가복점'수도중조자교계구건료SSR표기적유전도보.연합2007년화2008년획득적량조도미립장(GL)、립관(GW)、장관비(L/W)수거응용혼합선성모형방법진행QTL정위,병작가성효응、가성×가성상위호작효응이급가성QTL、상위성QTL여배경적호작효응분석.결과현시;(1)재가성효응분석중량개군체공검측도4개공제립장적QTL,4개공제립관적QTL,5개공제장관비적QTL,공헌솔분별위13.81%、15.36%화 16.29%.(2)재상위호작효응분석중량개군체공검측도2대공제립장적호작QTL,1대공제립관적호작QTL,3대공제장관비적호작QTL,공헌솔분별위5.77%、2.59%화7.42%.(3)배경호작검측중,발현공유13개가성QTL화4대QTL적가성×가성상위성여배경산생료호작효응.결과표명,상위성효응화가성효응도영향도미립형유전,QE호작효응야대립형유착현저적영향.
In this study,a recombinant inbred line (RIL) population derived from the indica-indica rice cross 'Guangluai 4'×'Jiafuzhan' was used in mapping of Quantitative trait loci (QTLs).Based on mixed linear model QTLmapper1.6,mapping was carried out for grain shape such as grain length (GL),grain width (GW) and length-width ratio (L/W) in rice in 2007 and 2008.QTLs were determined at the one-locus and two-locus levels,and QTL-by-environment (QE) interactions were analyzed.Four,four and five QTLs were detected to have significant additive effects for GL,GW and L/W,and the contribution rate were 13.81%,15.36% and 16.29%,respectively.Two,one and three pairs of epistatic QTLs with significant additive-by-additive (AA) interaction effects (epistatic effects) were identified for the three traits,and the contribution rate were 5.77%,2.59% and 7.42%,respectively.Significant QE interactions were found for thirteen QTLs with additive effects and four pairs of epistatic QTLs.The results indicated that the epistatic and additive effects played an important role on the inheritance of grain shape,and the environmental factor had significant effects on the three traits.