CAJ | 학술논문

[目的]为了获得在某些方面性能优良(如耐高温、耐强酸、耐强碱等)的α-淀粉酶产生菌.[方法]对α-淀粉酶生菌进行分离筛选,并对其酶学性质进行了研究.[结果]从稀释样品涂布的淀粉筛选平板上筛选出10株有明显淀粉水解圈的单个菌株,从中又测定得到3株α-淀粉酶酶活力较高的菌株即为:X6、X8和X10.这3株菌的最适pH值都在中性范围内,最适温度均为60℃.Ca2+能提高酶的热稳定性,X6、X8在Ca2+浓度为0.02～0.04 mol/L时,酶的热稳定性最高,X10在Ca2+浓度为0.03～0.04 mol/L时,酶的热稳定性最高;当Ca2+浓度继续增大时,酶的热稳定性反而降低.[结论]该研究为满足不同行业对不同特征的α-淀粉酶的需求提供了理论依据.
[목적]위료획득재모사방면성능우량(여내고온、내강산、내강감등)적α-정분매산생균.[방법]대α-정분매생균진행분리사선,병대기매학성질진행료연구.[결과]종희석양품도포적정분사선평판상사선출10주유명현정분수해권적단개균주,종중우측정득도3주α-정분매매활력교고적균주즉위:X6、X8화X10.저3주균적최괄pH치도재중성범위내,최괄온도균위60℃.Ca2+능제고매적열은정성,X6、X8재Ca2+농도위0.02～0.04 mol/L시,매적열은정성최고,X10재Ca2+농도위0.03～0.04 mol/L시,매적열은정성최고;당Ca2+농도계속증대시,매적열은정성반이강저.[결론]해연구위만족불동행업대불동특정적α-정분매적수구제공료이론의거.
[Objective]The aim was to obtain α-amylase producing strains with some excellent properties like high temperature resistance,strong acid resistance,strong alkali resistance,etc..[Method]α-amylase producing strains were isolated and screened;furthermore their enzymatic properties were studied.[Result]10 strains with an obvious starch hydrolysis cycle were screened out from starch screening plate coated by diluted sample,from which 3 strains with higher α-amylase activity were screened out,that was ×6,×8 and ×10.As for ×6,×8 and ×10,their optimum pH values all belonged to neutral,and their optimum temperatures were all 60℃.Meanwhile,Ca2+ could increase their enzyme thermal stability.When the concentration of Ca2+ was 0.02-0.04 mol/L,the enzyme thermal stability of ×6 and ×8 reached the highest;When the concentration of Ca2+ was 0.03-0.04 mol/L,that of ×10 reached the highest;When the concentration of Ca2+ was increased continuously,those of the 3 strains all decreased.[Conclusion]The research provides theoretical basis for satisfying the demands of different industries for α-amylase with different characteristics.