CAJ | 학술논문

目的探讨支气管哮喘(简称哮喘)大鼠肺内非肾上腺素能非胆碱能(non-adrenergic non-cholinergic,NANC)神经分布及其神经肽含量的变化并分析两者的相关性.方法 20只Wister大鼠随机分成正常组和哮喘组.采用免疫组织化学和计算机图象分析观察大鼠肺内降钙素基因相关肽(calcitonin gene-related peptide,CGRP)和血管活性肠肽(vasoactive intestinal peptide,VIP)免疫反应阳性纤维的变化,并且用酶联免疫吸附试验法测定肺泡灌洗液中CGRP和VIP的浓度.结果 ①哮喘大鼠肺内CGRP和VIP阳性纤维的分布和密度均发生了显著的变化,CGRP阳性纤维显著增多,染色加深,相对阳性染色面积和光密度值,与对照组相比,差异有统计学意义(P＜0.01);VIP阳性纤维明显缺乏,相对阳性染色面积和光密度值,与对照组相比,差异有统计学意义(P＜0.05或P＜0.01).②哮喘大鼠肺泡灌洗液中CGRP的浓度与正常对照组相比显著增高(P＜0.01),与CGRP阳性纤维的相对染色面积(r=0.903)和光密度值(r=0.880)呈正相关;VIP的浓度显著降低(P＜0.01),与VIP阳性纤维的相对染色面积(r=0.899)和光密度值(r=0.878)呈正相关.结论哮喘大鼠肺内NANC神经分布异常,这可能是导致相应的神经肽含量变化的直接原因.
목적 탐토지기관효천(간칭효천)대서폐내비신상선소능비담감능(non-adrenergic non-cholinergic,NANC)신경분포급기신경태함량적변화병분석량자적상관성.방법 20지Wister대서수궤분성정상조화효천조.채용면역조직화학화계산궤도상분석관찰대서폐내강개소기인상관태(calcitonin gene-related peptide,CGRP)화혈관활성장태(vasoactive intestinal peptide,VIP)면역반응양성섬유적변화,병차용매련면역흡부시험법측정폐포관세액중CGRP화VIP적농도.결과 ①효천대서폐내CGRP화VIP양성섬유적분포화밀도균발생료현저적변화,CGRP양성섬유현저증다,염색가심,상대양성염색면적화광밀도치,여대조조상비,차이유통계학의의(P＜0.01);VIP양성섬유명현결핍,상대양성염색면적화광밀도치,여대조조상비,차이유통계학의의(P＜0.05혹P＜0.01).②효천대서폐포관세액중CGRP적농도여정상대조조상비현저증고(P＜0.01),여CGRP양성섬유적상대염색면적(r=0.903)화광밀도치(r=0.880)정정상관;VIP적농도현저강저(P＜0.01),여VIP양성섬유적상대염색면적(r=0.899)화광밀도치(r=0.878)정정상관.결론 효천대서폐내NANC신경분포이상,저가능시도치상응적신경태함량변화적직접원인.
Objective To investigate the change of distribution of non-adrenergic non-cholinergic nerve and neuropeptides in the lung of asthmatic rat.Methods Twenty Wistar rats were divided into asthma group and control group.Immunohistochemistry and computer image analysis were used to observe the change of the calcitonin gene-related peptide(CGRP)and vasoactive intestinal peptide(VIP)immunoreactive (IR)nerve fibers.The contents of CGRP and VIP in lung tissues were detected by ELISA.Results ①There were significant changes in distribution and density of CGRP-IR and VIP-IR nerve fibers in airways of asthma group.The relative positive staining areas and mean optical density(MOD)of CGRP-IR nerve fibers increased significantly in asthma group compared with those of control group(P＜0.01).But the relative positive staining areas and MOD of VIP-IR nerve fibers decreased significantly in asthma group compared with those of control group(P＜0.05 or P＜0.01).②The content of CGRP in the BALF of asthmatic rats was significantly higher than that in control group.There was a significantly positive correlation between CGRP and the relative positive staining areas(r=0.903)and MOD(r=0.880)of CGRP-IR nerve fibers.The contents of VIP in the BALF of asthmatic rats were significantly lower than those in control group.There was a significantly positive correlation between VIP and the relative positive staining areas(r=0.899)and MOD(r=0.878)of VIP-IR nerve fibers.Conclusions The distribution of non-adrenergic non-cholinergic nerve were abnormal in the lung of asthmatic rats,which might immediately cause the change of neuropeptides.