白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2010年
7期
418-420
,共3页
多发性骨髓瘤%骨髓%成纤维样细胞%受体,CXCR4%CD49d%整合素α4
多髮性骨髓瘤%骨髓%成纖維樣細胞%受體,CXCR4%CD49d%整閤素α4
다발성골수류%골수%성섬유양세포%수체,CXCR4%CD49d%정합소α4
Multiple myeloma%Bone marrow%Fibroblasts%Receptors,CXCR4%CD49d%Integria alpha 4
目的 观察人类骨髓成纤维样细胞系HFCL对多发性骨髓瘤细胞RPMI8226增殖、迁移和归巢的影响.方法 采用体外细胞培养,建立RPMI8226细胞和HFCL细胞共培养体系,锥虫蓝拒染法测定生长曲线;流式细胞术(FCM)检测细胞周期和黏附分子CD49d的变化;RT-PCR检测CXCR4基因的表达情况.结果 HFCL细胞抑制RPMI8226细胞生长,且与HFCL细胞直接接触组的抑制作用大于非直接接触组(Transwell)组.RPMI8226细胞与HFCL细胞共培养后,直接接触组G1期细胞增高,S期细胞减少:HFCL细胞下调RPMI8226细胞的CXCR4和CD49d 的表达,单独培养组明显高于直接培养组;Transwell组无明显变化.结论 人类骨髓成纤维样细胞HFCL能抑制多发性骨髓瘤细胞RPMI8226的增殖,抑制CXCR4和CD49d的表达,影响骨髓瘤细胞的迁移和归巢.
目的 觀察人類骨髓成纖維樣細胞繫HFCL對多髮性骨髓瘤細胞RPMI8226增殖、遷移和歸巢的影響.方法 採用體外細胞培養,建立RPMI8226細胞和HFCL細胞共培養體繫,錐蟲藍拒染法測定生長麯線;流式細胞術(FCM)檢測細胞週期和黏附分子CD49d的變化;RT-PCR檢測CXCR4基因的錶達情況.結果 HFCL細胞抑製RPMI8226細胞生長,且與HFCL細胞直接接觸組的抑製作用大于非直接接觸組(Transwell)組.RPMI8226細胞與HFCL細胞共培養後,直接接觸組G1期細胞增高,S期細胞減少:HFCL細胞下調RPMI8226細胞的CXCR4和CD49d 的錶達,單獨培養組明顯高于直接培養組;Transwell組無明顯變化.結論 人類骨髓成纖維樣細胞HFCL能抑製多髮性骨髓瘤細胞RPMI8226的增殖,抑製CXCR4和CD49d的錶達,影響骨髓瘤細胞的遷移和歸巢.
목적 관찰인류골수성섬유양세포계HFCL대다발성골수류세포RPMI8226증식、천이화귀소적영향.방법 채용체외세포배양,건립RPMI8226세포화HFCL세포공배양체계,추충람거염법측정생장곡선;류식세포술(FCM)검측세포주기화점부분자CD49d적변화;RT-PCR검측CXCR4기인적표체정황.결과 HFCL세포억제RPMI8226세포생장,차여HFCL세포직접접촉조적억제작용대우비직접접촉조(Transwell)조.RPMI8226세포여HFCL세포공배양후,직접접촉조G1기세포증고,S기세포감소:HFCL세포하조RPMI8226세포적CXCR4화CD49d 적표체,단독배양조명현고우직접배양조;Transwell조무명현변화.결론 인류골수성섬유양세포HFCL능억제다발성골수류세포RPMI8226적증식,억제CXCR4화CD49d적표체,영향골수류세포적천이화귀소.
Objective To investigate the effects of human bone marrow fibroblastoid stromal cell lines HFCL on the proliferation .migrating and homing of multiple myeloma cell lines RPM18226. Methods The co-culture system of RPMI8226 and HFCL was established through cell culture in vitro, growth curves were determined by trypan blue exclusion, cell cycle and expression of adhension molecule CD49d were detected by flow cytometry, and expression of CXCR4 gene was examined by RT-PCR. Results The proliferation of RPMI8226 cells in direct contact with HFCL cells group was inhibited strongerly than that in transwell group. The percentage of G1 phase cells of RPMI8226 cells in direct contact with HFCL group was higher than that of RPM 18226 in transwell group, while the percentage of S phase cells was lower. The expressions of CD49d and CXCR4 in RPMI8226 cells were down-regulated by HFCL cells, and that in transwell group was higher than that in direct contact with HFCL cells group. Conclusion Human bone marrow fibroblastoidss tromal cell HFCL can inhibit the proliferation and the expressions of CD49d and CXCR4 of multiple myeloma cell line RPMI8226, and can prevent multiple myeloma cells from migrating and homing.
