CAJ | 학술논문

目的比较大鼠蛛网膜下腔长期置管和反复蛛网膜下腔穿刺两种给药方法的难易程度及反复给药的可行性.方法 40只清洁级SD大鼠以随机数字表法分为置管组和穿刺组各20只.置管组通过蛛网膜下腔长期置管给药,穿刺组反复行蛛网膜下腔穿刺给药.记录每次进行操作所用的时间.2组均从实验第5天开始鞘内注射吗啡10 μg/1μl,连续给药7 d,用热辐射法测定大鼠热缩足潜伏期(TWL).对2组实验时间和TWL进行比较分析.结果置管组操作时间较穿刺组短,差异有统计学意义(P<0.01).置管组5只大鼠导管脱出.鞘内给药后,置管组和穿刺组TWL测定值均较基础值增加,差异有统计学意义(P<0.01).2组间TWL测定值差异无统计学意义.结论蛛网膜下腔长期置管和反复蛛网膜下腔穿刺均可作为鞘内多次给药方法,但在选择时应该考虑到操作难易程度、给药方式、反复给药并发症等因素的影响.
목적 비교대서주망막하강장기치관화반복주망막하강천자량충급약방법적난역정도급반복급약적가행성.방법 40지청길급SD대서이수궤수자표법분위치관조화천자조각20지.치관조통과주망막하강장기치관급약,천자조반복행주망막하강천자급약.기록매차진행조작소용적시간.2조균종실험제5천개시초내주사마배10 μg/1μl,련속급약7 d,용열복사법측정대서열축족잠복기(TWL).대2조실험시간화TWL진행비교분석.결과 치관조조작시간교천자조단,차이유통계학의의(P<0.01).치관조5지대서도관탈출.초내급약후,치관조화천자조TWL측정치균교기출치증가,차이유통계학의의(P<0.01).2조간TWL측정치차이무통계학의의.결론 주망막하강장기치관화반복주망막하강천자균가작위초내다차급약방법,단재선택시응해고필도조작난역정도、급약방식、반복급약병발증등인소적영향.
Objective To compare the feasibility of direct spinal puncture and reserving micro-catheter in spinal space during repeated intrathecal administration. Methods Forty Sprague-Dawley rats were randomly divided into 2 groups:direct spinal puncture (group puncture) and reserving micro-catheter in spinal space group (group reserve). The duration of operation were recorded. 5 days after operation, rata in both groups were intrathecally administerde morphine(10 μg/10μl) for 7 days and thermal withdrawal latency(TWL) were assessed. Results The operation in group reserve took shorter time than the group puncture, but micro-catheter were pulled out in 5 rats in group reserve. There is no difference of TWL between two groups. Conclusion Both methods are suitable for repeated intrathecal administration.