CAJ | 학술논문

目的探讨锰超氧化物歧化酶(MnSOD)基因多态性与职业性慢性锰中毒易感性的关系.方法采用1∶2配对病例-对照研究的方法,应用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)分析与比较了164例汉族职业性慢性锰中毒病例与328例汉族对照的MnSOD基因9Ala-Val位点的基因多态性,分析MnSOD基因多态性与职业性慢性锰中毒的关系.结果 MnSOD基因9AlaVal位点V等位基因在对照组和病例组中的分布频率分别为76.7%和87.2%,差异有统计学意义(x2=15.225,P＜0.01,OR=2.07,95%CI=1.43～3.00);具有VV基因型的个体发生职业性慢性锰中毒的危险性与具有非VV基因型的个体相比升高了1.30倍(OR=2.30,95%CI=1.52～3.49,P＜0.05).结论 MnSOD基因可能是职业性慢性锰中毒的易感基因,携带MnSOD基因型VV可能增加职业性慢性锰中毒的危险性.
목적 탐토맹초양화물기화매(MnSOD)기인다태성여직업성만성맹중독역감성적관계.방법 채용1∶2배대병례-대조연구적방법,응용취합매련반응-한제성편단장도다태성방법(PCR-RFLP)분석여비교료164례한족직업성만성맹중독병례여328례한족대조적MnSOD기인9Ala-Val위점적기인다태성,분석MnSOD기인다태성여직업성만성맹중독적관계.결과 MnSOD기인9AlaVal위점V등위기인재대조조화병례조중적분포빈솔분별위76.7%화87.2%,차이유통계학의의(x2=15.225,P＜0.01,OR=2.07,95%CI=1.43～3.00);구유VV기인형적개체발생직업성만성맹중독적위험성여구유비VV기인형적개체상비승고료1.30배(OR=2.30,95%CI=1.52～3.49,P＜0.05).결론 MnSOD기인가능시직업성만성맹중독적역감기인,휴대MnSOD기인형VV가능증가직업성만성맹중독적위험성.
Objective To study the relationship between polymorphisms of MnSOD and the susceptibility of chronic poisoning exposed to manganism occupationally. Methods In a study of case-control,genotypes were determined by PCR-RFLP in 164 patients with chronic occupational mangamism poisoning and 328 controls with age- and sex-matched for MnSOD 9Ala-Val. Results There was a significant difference in the frequency of MnSOD 9Ala-Val at V locus mutant allele between cases and controls(x2=15.225,P＜0.01,95%CI=1.43～3.00).Individuals with the genotype VV had a 1.30 of risk increase of occupational chronic manganism poisoning compared with the the genotype AV or AA (OR=2.30,95%CI=1.52～3.49,P＜0.05). Conclusion The MnSOD polymorphisms may be related with the susceptibility to chronic occupational manganism poisoning,the risk of chronic occupational manganism poisoning increases in carriers with genotype VV at MnSOD 9AlaVal locus.