中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
7期
1054-1056
,共3页
沈为栋%尚雷%刘岸%沈跃%廖维%胡丽萍%黄莉莉
瀋為棟%尚雷%劉岸%瀋躍%廖維%鬍麗萍%黃莉莉
침위동%상뢰%류안%침약%료유%호려평%황리리
百里醌%骨肉瘤%脱噬作用
百裏醌%骨肉瘤%脫噬作用
백리곤%골육류%탈서작용
Thymoquinone%Osteosarcoma%Apoptosis
目的 探讨百里醌对体外人骨肉瘤SaOS-2细胞生长的影响及其机制.方法 百里醌作用人骨肉瘤SaOS-2细胞24h后,细胞计数CCK-8法检测细胞增殖;流式细胞仪检测细胞凋亡;DAPI染色后荧光显微镜下观察细胞形态学变化;Western blot检测SaOS-2细胞中Caspase-3、X连锁凋亡抑制蛋白(XIAP)和Smac的表达.结果 不同浓度(20、40、80μmol/L)百里醌作用人骨肉瘤SaOS-2细胞24h后,细胞存活率分别为(75.5±4.2)%、(62.1±6.7)%和(52.5±4.9)%;细胞早期凋亡率分别为(8.1±0.7)%、(13.2±1.1)%和(20.2±1.7)%;百里醌作用后,SaOS-2细胞出现典型凋亡的形态学改变;百里醌可明显上调Caspase-3和Smac在SaOS-2细胞中的表达,而显著抑制XIAP的表达.结论 百里醌具有抑制体外人骨肉瘤SaOS-2细胞生长和促进细胞凋亡的作用,可能是通过上调人骨肉瘤SaOS-2细胞中Caspase-3和Smac的表达和下调XIAP的表达而实现.
目的 探討百裏醌對體外人骨肉瘤SaOS-2細胞生長的影響及其機製.方法 百裏醌作用人骨肉瘤SaOS-2細胞24h後,細胞計數CCK-8法檢測細胞增殖;流式細胞儀檢測細胞凋亡;DAPI染色後熒光顯微鏡下觀察細胞形態學變化;Western blot檢測SaOS-2細胞中Caspase-3、X連鎖凋亡抑製蛋白(XIAP)和Smac的錶達.結果 不同濃度(20、40、80μmol/L)百裏醌作用人骨肉瘤SaOS-2細胞24h後,細胞存活率分彆為(75.5±4.2)%、(62.1±6.7)%和(52.5±4.9)%;細胞早期凋亡率分彆為(8.1±0.7)%、(13.2±1.1)%和(20.2±1.7)%;百裏醌作用後,SaOS-2細胞齣現典型凋亡的形態學改變;百裏醌可明顯上調Caspase-3和Smac在SaOS-2細胞中的錶達,而顯著抑製XIAP的錶達.結論 百裏醌具有抑製體外人骨肉瘤SaOS-2細胞生長和促進細胞凋亡的作用,可能是通過上調人骨肉瘤SaOS-2細胞中Caspase-3和Smac的錶達和下調XIAP的錶達而實現.
목적 탐토백리곤대체외인골육류SaOS-2세포생장적영향급기궤제.방법 백리곤작용인골육류SaOS-2세포24h후,세포계수CCK-8법검측세포증식;류식세포의검측세포조망;DAPI염색후형광현미경하관찰세포형태학변화;Western blot검측SaOS-2세포중Caspase-3、X련쇄조망억제단백(XIAP)화Smac적표체.결과 불동농도(20、40、80μmol/L)백리곤작용인골육류SaOS-2세포24h후,세포존활솔분별위(75.5±4.2)%、(62.1±6.7)%화(52.5±4.9)%;세포조기조망솔분별위(8.1±0.7)%、(13.2±1.1)%화(20.2±1.7)%;백리곤작용후,SaOS-2세포출현전형조망적형태학개변;백리곤가명현상조Caspase-3화Smac재SaOS-2세포중적표체,이현저억제XIAP적표체.결론 백리곤구유억제체외인골육류SaOS-2세포생장화촉진세포조망적작용,가능시통과상조인골육류SaOS-2세포중Caspase-3화Smac적표체화하조XIAP적표체이실현.
Objective To investigate the inhibitory effect of thymoquinone on the growth of human osteosarcoma cell line SaOS-2 in vitro.Methods After human osteosarcoma SaOS-2 cells were treated with different concentrations of thymoquinone, the proliferation was measured by Cell Counting Kit-8 (CCK-8) assay. The flowcytometry (FCM) was used to determine apoptosis of SaOS-2 cells. The morphological changes of SaOS-2 cells were observed under the fluorescence microscopy after DAPI staining. Western blotting was used to detect the protein expression of Caspase-3, X-linked inhibitor of apoptosis protein (XIAP) and Smac.Results Thymoquinone obviously suppressed proliferation of SaOS-2 cells in a dose-dependent manner, and the apoptosis rate was (8.1±0.7)%, (13.2±1.1)% and (20.2±1.7)% respectively when the concentrations of thymoquinone exposed were 20, 40 and 80 μmol/L. After treatment with thymoquinone, the expression of Caspase-3 and Smac was up-regulated in SaOS-2 cells, and thymoquinone treatment significantly decreased the XIAP levels in SaOS-2 cells.Conclusion Thymoquinone has the anti-tumor effect on the human osteosarcoma SaOS-2 cells in vitroprobably by up-regulating the expression of Caspase-3 and Smac and down-regulating the expression of XIAP.
