CAJ | 학술논문

目的观察兔视网膜下非活动载片植入后视网膜相关蛋白的表达.方法健康成年新西兰白兔27只,随机分为手术组、伪手术组及对照组,3组分别为12、12、3只兔.手术组和伪手术组再分为手术后7、15、30、60 d亚组,每组随机分配3只兔,手术组为非活动载片植入,伪手术组非活动载片植入随即取出.于手术后7、15、30、60 d处死动物后进行免疫组织化学染色,对照组于饲养30 d后处死动物行免疫组织化学染色,观察胶质纤维酸性蛋白(GFAP)和脑源性神经营养因子(BDNF)在视网膜的表达.结果手术组手术后7、15、30 d组载片上外层视网膜明显变薄,内层细胞排列紊乱;60 d组胶质细胞增生,手术组载片上视网膜GFAP和BDNF阳性表达均较伪手术组和对照组增加,伪手术组GFAP和BDNF阳性表达均较对照组增加,各组内各时间点间GFAP和BDNF表达未见明显差异.结论载片植入后局部视网膜神经保护相关蛋白表达升高,提示载片植入可能有神经保护作用参与,但其作用有限.
목적 관찰토시망막하비활동재편식입후시망막상관단백적표체.방법 건강성년신서란백토27지,수궤분위수술조、위수술조급대조조,3조분별위12、12、3지토.수술조화위수술조재분위수술후7、15、30、60 d아조,매조수궤분배3지토,수술조위비활동재편식입,위수술조비활동재편식입수즉취출.우수술후7、15、30、60 d처사동물후진행면역조직화학염색,대조조우사양30 d후처사동물행면역조직화학염색,관찰효질섬유산성단백(GFAP)화뇌원성신경영양인자(BDNF)재시망막적표체.결과 수술조수술후7、15、30 d조재편상외층시망막명현변박,내층세포배렬문란;60 d조효질세포증생,수술조재편상시망막GFAP화BDNF양성표체균교위수술조화대조조증가,위수술조GFAP화BDNF양성표체균교대조조증가,각조내각시간점간GFAP화BDNF표체미견명현차이.결론 재편식입후국부시망막신경보호상관단백표체승고,제시재편식입가능유신경보호작용삼여,단기작용유한.
Objective To observe the expression of related proteins of retina after subretinal implantation with inactive chips.Methods A total of 27 healthy adult New Zealand white rabbits were randomly divided into three groups:operation group (12 rabbits) in which the rabbits were implanted with inactive chips into the interspace beneath retina;sham-operation group (12 rabbits) in which the rabbits were implanted with inactive chips into the interspace beneath retina which was taken out immediately;the control group (3 rabbits).Animals were sacrified for immunohistological study 7,15,30 and 60 days after surgery.The rabbits in control group group were sacrified for immunohistological study after bred for 30 days.The expressions of glial fibrillary acidic protein (GFAP) and brain derived neurotrophic facor (BDNF) were observed.Results In operation group,the outer nulear layer of retina thinned,and the cells in the inner nulear layer was disorganized 7,15,and 30 days after the surgery;glial cells proliferated 60 days after surgery;the positive expression of BDNF and GFAP was more than that in the sham-operation and control group.In sham-operation group,the positive expression of BDNF and GFAP was more than that in the control group.No obvious difference of expression of BDNF and GFAP between each time point groups was found.Conclusions The-expression of neroprotective related proteins increased after subretinal implantation with inactive chips suggests that limited neuroprotective effects might be led by the implantation.