中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2010年
3期
166-170
,共5页
刘雷%陈卫昌%史冬涛%宋振云%郜恒骏%冯砅锦%王维%沈晓莹
劉雷%陳衛昌%史鼕濤%宋振雲%郜恆駿%馮砅錦%王維%瀋曉瑩
류뢰%진위창%사동도%송진운%고항준%풍례금%왕유%침효형
胃肿瘤%组织芯片%抑癌基因
胃腫瘤%組織芯片%抑癌基因
위종류%조직심편%억암기인
Stomach neoplasms Tissue mieroarray%Genes,suppressor
目的 研究胃腺癌及非癌组织中Runx3、Smad4和p21的表达及临床意义.方法 收集130例胃腺癌患者手术切除标本制作的石蜡块共378份,其中癌组织130份,癌旁组织和非癌组织248份,每份组织制成4块组织芯片,分别为174点、282点、156点和156点.采用免疫组化法检测其中Runx3、Smad4和p2l的表达.结果 Runx3在胃腺癌中的异常表达率高于非癌组织(P<0.05),Smad4及p2l的异常表达在胃黏膜癌变过程中呈上升趋势(P<0.05).Runx3的异常表达与胃腺癌组织学分化程度和淋巴结转移有关(P<0.05),Smad4和p21的异常表达均与胃腺癌的分化程度相关(P<0.05).胃腺癌中Runx3表达与Smad4和p21表达呈正相关(r=0.387,P<0.05;r=0.840,P<0.05).Runx3、Smad4、p21正常表达患者的3年生存率均大于异常表达患者(P<0.05).多因素分析显示,Runx3和Smad4的表达可作为胃腺癌患者独立的预后因素(P<0.05).结论 Runx3、Smad4和p21在胃腺癌的发生、发展中可能起一定的作用.Runx3和Smad4可作为判断胃腺癌患者预后的指标之一.
目的 研究胃腺癌及非癌組織中Runx3、Smad4和p21的錶達及臨床意義.方法 收集130例胃腺癌患者手術切除標本製作的石蠟塊共378份,其中癌組織130份,癌徬組織和非癌組織248份,每份組織製成4塊組織芯片,分彆為174點、282點、156點和156點.採用免疫組化法檢測其中Runx3、Smad4和p2l的錶達.結果 Runx3在胃腺癌中的異常錶達率高于非癌組織(P<0.05),Smad4及p2l的異常錶達在胃黏膜癌變過程中呈上升趨勢(P<0.05).Runx3的異常錶達與胃腺癌組織學分化程度和淋巴結轉移有關(P<0.05),Smad4和p21的異常錶達均與胃腺癌的分化程度相關(P<0.05).胃腺癌中Runx3錶達與Smad4和p21錶達呈正相關(r=0.387,P<0.05;r=0.840,P<0.05).Runx3、Smad4、p21正常錶達患者的3年生存率均大于異常錶達患者(P<0.05).多因素分析顯示,Runx3和Smad4的錶達可作為胃腺癌患者獨立的預後因素(P<0.05).結論 Runx3、Smad4和p21在胃腺癌的髮生、髮展中可能起一定的作用.Runx3和Smad4可作為判斷胃腺癌患者預後的指標之一.
목적 연구위선암급비암조직중Runx3、Smad4화p21적표체급림상의의.방법 수집130례위선암환자수술절제표본제작적석사괴공378빈,기중암조직130빈,암방조직화비암조직248빈,매빈조직제성4괴조직심편,분별위174점、282점、156점화156점.채용면역조화법검측기중Runx3、Smad4화p2l적표체.결과 Runx3재위선암중적이상표체솔고우비암조직(P<0.05),Smad4급p2l적이상표체재위점막암변과정중정상승추세(P<0.05).Runx3적이상표체여위선암조직학분화정도화림파결전이유관(P<0.05),Smad4화p21적이상표체균여위선암적분화정도상관(P<0.05).위선암중Runx3표체여Smad4화p21표체정정상관(r=0.387,P<0.05;r=0.840,P<0.05).Runx3、Smad4、p21정상표체환자적3년생존솔균대우이상표체환자(P<0.05).다인소분석현시,Runx3화Smad4적표체가작위위선암환자독립적예후인소(P<0.05).결론 Runx3、Smad4화p21재위선암적발생、발전중가능기일정적작용.Runx3화Smad4가작위판단위선암환자예후적지표지일.
Objective To investigate the expressions of Runx3,Smad4 and p21 in human gastric adenocarcinoma and non-cancerous tissues and the clinical significance.Methods Three hundred and seventy eight paraffin embedded blocks prepared from surgical samples of 130 patients with gastric adenocarcinoma consisted of 130 cancerous blocks and 248 non-cancerous blocks.Four tissue microarrays (174 dots,282 dots,156 dots and 156 dots) were made for each block.The expressions of Runx3,Smad4 and p21 were detected by immunohistochemistry.Results The abnormal expression of Runx3 was higher in cancerous tissues than in non-cancerous tissues (P<0.05).The aberrant expressions of Smad4 and p21 were increased gradually in the process of gastric carcinogenesis.The aberrant expression of Runx3 was associated with tumor differentiation and lymph node metastasis (P<0.05).Whereas the expressions of Smad4 and p21 were closely related to the tumor differentiation (P<0.05).The aberrant expression of Runx3 was positively correlated with the abnormal expressions of Smad4 and p21 (r=0.387,P<0.05;r=0.840,P<0.05,respectively). Kaplan-Meier analysis showed that the 3-year survival rate in patients with normal expressions of Runx3,Smad4 and p21 was significantly higher than that in patients with abnormal expressions (P<0.05).Furthermore,multivariate analysis indicated that Runx3 and Smad4 expressions were independent prognostic factors for patients with gastric cancer(P<0.05).Conclusions The Runx3,Smad4 and p21 may involve in the carcinogenesis of gastric adenocarcinoma.Runx3 or Smad4 may be one of the index in prognosis of patients with gastric adenoearcinoma.
