南京师大学报(自然科学版)
南京師大學報(自然科學版)
남경사대학보(자연과학판)
JOURNAL OF NANJING NORMAL UNIVERSITY(NATURAL SCIENCE)
2001年
1期
95-100
,共6页
施旭东%范必勤%王公金%曹步凯%陈杰%谭笑梅%陈华%孙贝加%王金锋%糜祖煌
施旭東%範必勤%王公金%曹步凱%陳傑%譚笑梅%陳華%孫貝加%王金鋒%糜祖煌
시욱동%범필근%왕공금%조보개%진걸%담소매%진화%손패가%왕금봉%미조황
人%FSH%腔前卵泡%体外培养%E2
人%FSH%腔前卵泡%體外培養%E2
인%FSH%강전란포%체외배양%E2
为探讨FSH对人腔前卵泡体外生长的影响，为此通过在培养液中加入不同浓度的FSH，观察卵泡存活天数和卵泡发育增大，并测定卵泡E2的分泌量.结果发现：卵泡体外存活天数：对照组（无FSH）为2.80±1.69-d，Ⅰ、Ⅱ、Ⅲ组（FSH浓度为0.5-IU/mL、1.0-IU/mL、2.0-IU/mL）分别为5.36±0.63、5.47±2.50、8.13±4.19d，与对照组相比，分别为P＜0.05、P＜0.01、P＜0.001.卵泡增大比率：对照组为20.00%，Ⅰ、Ⅱ、Ⅲ组分别为35.70%、60.00%、81.25%，与对照组相比分别为P＞0.05、P＜0.05、P＜0.01.E2分泌量：对照组为49.94±20.91-pg/mL，Ⅰ、Ⅱ、Ⅲ组分别为108.63±41.06、315.53±93.39、438.24±187.61-pg/mL.对照组与Ⅰ组相比，P＜0.001；Ⅰ与Ⅱ组相比，P＜0.001；Ⅱ与Ⅲ组相比，P＜0.05.可见FSH能使卵泡体外存活天数延长，卵泡发育增大，卵泡E2分泌增加，并大致呈正相关关系.
為探討FSH對人腔前卵泡體外生長的影響，為此通過在培養液中加入不同濃度的FSH，觀察卵泡存活天數和卵泡髮育增大，併測定卵泡E2的分泌量.結果髮現：卵泡體外存活天數：對照組（無FSH）為2.80±1.69-d，Ⅰ、Ⅱ、Ⅲ組（FSH濃度為0.5-IU/mL、1.0-IU/mL、2.0-IU/mL）分彆為5.36±0.63、5.47±2.50、8.13±4.19d，與對照組相比，分彆為P＜0.05、P＜0.01、P＜0.001.卵泡增大比率：對照組為20.00%，Ⅰ、Ⅱ、Ⅲ組分彆為35.70%、60.00%、81.25%，與對照組相比分彆為P＞0.05、P＜0.05、P＜0.01.E2分泌量：對照組為49.94±20.91-pg/mL，Ⅰ、Ⅱ、Ⅲ組分彆為108.63±41.06、315.53±93.39、438.24±187.61-pg/mL.對照組與Ⅰ組相比，P＜0.001；Ⅰ與Ⅱ組相比，P＜0.001；Ⅱ與Ⅲ組相比，P＜0.05.可見FSH能使卵泡體外存活天數延長，卵泡髮育增大，卵泡E2分泌增加，併大緻呈正相關關繫.
위탐토FSH대인강전란포체외생장적영향，위차통과재배양액중가입불동농도적FSH，관찰란포존활천수화란포발육증대，병측정란포E2적분비량.결과발현：란포체외존활천수：대조조（무FSH）위2.80±1.69-d，Ⅰ、Ⅱ、Ⅲ조（FSH농도위0.5-IU/mL、1.0-IU/mL、2.0-IU/mL）분별위5.36±0.63、5.47±2.50、8.13±4.19d，여대조조상비，분별위P＜0.05、P＜0.01、P＜0.001.란포증대비솔：대조조위20.00%，Ⅰ、Ⅱ、Ⅲ조분별위35.70%、60.00%、81.25%，여대조조상비분별위P＞0.05、P＜0.05、P＜0.01.E2분비량：대조조위49.94±20.91-pg/mL，Ⅰ、Ⅱ、Ⅲ조분별위108.63±41.06、315.53±93.39、438.24±187.61-pg/mL.대조조여Ⅰ조상비，P＜0.001；Ⅰ여Ⅱ조상비，P＜0.001；Ⅱ여Ⅲ조상비，P＜0.05.가견FSH능사란포체외존활천수연장，란포발육증대，란포E2분비증가，병대치정정상관관계.
To investigate the effect of FSH on the growth of human preantralfollicles cultured in vitro.FSH with different concentrations was added to the medium.The survival time and growth of follicles were observed and the levels of E2 was measured.The survival time of follicles cultured in vitro were 2.80±1.69 days for the control group ,5.36±0.63,5.47±2.50 and 8.13±4.19 days forⅠ,Ⅱand Ⅲ groups with FSH 0.5-IU/mL,1.0-IU/mL and 2.0-IU/mL respectively. Compared with control group,the statistics test results were P＜0.05,P＜0.01 and P＜0.001 respectively.The rates of follicular diameter increasing were 20.00% for the control group,35.71%,60.00% and 81.25% forⅠ,Ⅱand Ⅲ groups respectively.Compared with control group,the statistics test results were P＞0.05,P＜0.05,P＜0.01 respectively.The amount of E2 secreted by follicles were 49.94±20.91-pg/mL for control group, 108.63±41.06 ,315.53±93.39 ,438.24±187.61-pg/mL for Ⅰ,Ⅱ，Ⅲ groups respectively.The statistics test results were P＜0.001,P＜0.001,P＜0.05 between control and Ⅰ groups,Ⅰ and Ⅱ groups,Ⅱ and Ⅲ groups respectively.By adding FSH,the survival time of follicles cultured in vitro was lengthened and the follicles were growing larger.The secretion of E2 was increased.The relation between them was approximately positive correlative.
