植物学报
植物學報
식물학보
ACTA BOTANICA SINICA
2004年
10期
1220-1225
,共6页
陈豫%李辉%李洪杰%李义文%朱银峰%梁辉%曲乐庆%贾旭
陳豫%李輝%李洪傑%李義文%硃銀峰%樑輝%麯樂慶%賈旭
진예%리휘%리홍걸%리의문%주은봉%량휘%곡악경%가욱
水稻谷蛋白GluA-2基因%小麦转化%营养品质改良
水稻穀蛋白GluA-2基因%小麥轉化%營養品質改良
수도곡단백GluA-2기인%소맥전화%영양품질개량
rice glutelin gene GluA-2%wheat transformation%nutrient quality improvement
水稻(Oryza sativa L.)谷蛋白(Glutelin)约占水稻储藏蛋白总量的80%,谷蛋白赖氨酸含量较高并易于被人体消化吸收.为了提高小麦(Triticum aestivum L.)的营养品质,将水稻谷蛋白GluA-2基因的cDNA序列导人小麦栽培品种Bobwhite(T. aestivum cv.Bobwhite).共轰击了600个小麦幼胚,经PCR和Southern杂交鉴定,共获得4棵转GluA-2基因小麦;SDS-PAGE分析表明,GluA-2基因在3棵转基因植株及其后代中表达,在1棵转基因植株中未表达,但其内源的高分子量麦谷蛋白亚基Bx7和By9含量显著降低,并且可遗传至T1代.
水稻(Oryza sativa L.)穀蛋白(Glutelin)約佔水稻儲藏蛋白總量的80%,穀蛋白賴氨痠含量較高併易于被人體消化吸收.為瞭提高小麥(Triticum aestivum L.)的營養品質,將水稻穀蛋白GluA-2基因的cDNA序列導人小麥栽培品種Bobwhite(T. aestivum cv.Bobwhite).共轟擊瞭600箇小麥幼胚,經PCR和Southern雜交鑒定,共穫得4棵轉GluA-2基因小麥;SDS-PAGE分析錶明,GluA-2基因在3棵轉基因植株及其後代中錶達,在1棵轉基因植株中未錶達,但其內源的高分子量麥穀蛋白亞基Bx7和By9含量顯著降低,併且可遺傳至T1代.
수도(Oryza sativa L.)곡단백(Glutelin)약점수도저장단백총량적80%,곡단백뢰안산함량교고병역우피인체소화흡수.위료제고소맥(Triticum aestivum L.)적영양품질,장수도곡단백GluA-2기인적cDNA서렬도인소맥재배품충Bobwhite(T. aestivum cv.Bobwhite).공굉격료600개소맥유배,경PCR화Southern잡교감정,공획득4과전GluA-2기인소맥;SDS-PAGE분석표명,GluA-2기인재3과전기인식주급기후대중표체,재1과전기인식주중미표체,단기내원적고분자량맥곡단백아기Bx7화By9함량현저강저,병차가유전지T1대.
Rice (Oryza sativa L.) glutelin accounts for about 80% of total seed storage protein. Glutelin can be easily digested by human and contains high concentrations of lysine and other essential amino acids. To improve the nutritional quality of wheat (Triticum aestivum L.), the cDNA sequence of rice glutelin gene GluA-2 was introduced into common wheat through biolistic bombardment. Six hundreds of immature embryos from wheat cultivar Bobwhite ( T. aestivum cv. Bobwhite) were bombarded. Four transgenic plants carrying GluA-2 gene were confirmed by PCR and Southern blotting analyses. Gene GluA-2 was translated in three of the transgenic plants and their progeny, as indicated by SDS-PAGE analyses. The expression of GluA-2 gene was not detected in one transgenic plant and the endogenous wheat high molecular weight glutenin subunits Bx7 and By9 contents decreased greatly in both To and T1 generations.