CAJ | 학술논문

以冀无2031为材料,采用正交设计方法,研究了农杆菌介导的棉花下胚轴遗传转化过程中菌液浓度、侵染时间、共培养时间和共培养温度对抗性愈伤转化效率的影响.结果表明,4个因素均对抗性愈伤的诱导率有极显著影响,其诱导效应依次是共培养温度>菌液OD值>侵染时间>共培养时间.按照正交设计的原则,最终得到农杆菌介导转化棉花下胚轴的最佳条件是:菌液OD值为0.3,侵染15 min,24 ℃共培养24 h,下胚轴抗性愈伤的转化效率最高.用珂312、中521和农大94-7对所选出的最佳组合进行了进一步验证,结果3个品种在优选组合下的抗性愈伤组织诱导率都很高,分别为93.85%、86.72%和85.83%.
이기무2031위재료,채용정교설계방법,연구료농간균개도적면화하배축유전전화과정중균액농도、침염시간、공배양시간화공배양온도대항성유상전화효솔적영향.결과표명,4개인소균대항성유상적유도솔유겁현저영향,기유도효응의차시공배양온도>균액OD치>침염시간>공배양시간.안조정교설계적원칙,최종득도농간균개도전화면화하배축적최가조건시:균액OD치위0.3,침염15 min,24 ℃공배양24 h,하배축항성유상적전화효솔최고.용가312、중521화농대94-7대소선출적최가조합진행료진일보험증,결과3개품충재우선조합하적항성유상조직유도솔도흔고,분별위93.85%、86.72%화85.83%.
The influences of Agrobacterium concentration,infection time,the time and the temperature of co-cultute on the induction ratios of kanamycin-resistant calli were studied by Agrobacterium -mediated transformation using the hypocotyls of Jiwu 2031 as explants.The factors were optimized using orthogonal design.The results showed that the transformation ratio was the highest under the regime of OD=0.3 of bacteria concentration,15 min of infection time,24 ℃ of co-culture temperature and 24 h of co-culture time.High frequency of kanamycin-resistant calli formation was observed when infecting hypocotyls of Coker 312,Zhong 521 and Nongda 94-7 using the optimized combination of factors,and the induction ratios reached 93.85%,86.72% and 85.83%,respectively.