CAJ | 학술논문

采用UV光谱法、荧光光谱法,在pH=7.40的缓冲溶液中确定了镝(Ⅲ)与色氨酸的结合比n_(Dy(Ⅲ)):n_(Trp)=1:3,Dy(Ⅲ)(Trp)_3配合物与鲱鱼精DNA的结合比n_(Dy(Ⅲ)(Trp)_3):n_(DNA)=2:1.用双倒数法确定了结合常数K_(25℃)=5.75×10~4 L·mol~(-1)和K_(37℃)=3.27×10~4 L·mol~(-1).化学热力学研究显示配合物ny(Ⅲ)(Trp)_3与hsDNA的结合为熵和焓共同驱动.结合Scatchard法和粘度法,确定了配合物Dy(Ⅲ)(Trp)_3与hsDNA之间主要为静电作用和嵌插作用.
채용UV광보법、형광광보법,재pH=7.40적완충용액중학정료적(Ⅲ)여색안산적결합비n_(Dy(Ⅲ)):n_(Trp)=1:3,Dy(Ⅲ)(Trp)_3배합물여비어정DNA적결합비n_(Dy(Ⅲ)(Trp)_3):n_(DNA)=2:1.용쌍도수법학정료결합상수K_(25℃)=5.75×10~4 L·mol~(-1)화K_(37℃)=3.27×10~4 L·mol~(-1).화학열역학연구현시배합물ny(Ⅲ)(Trp)_3여hsDNA적결합위적화함공동구동.결합Scatchard법화점도법,학정료배합물Dy(Ⅲ)(Trp)_3여hsDNA지간주요위정전작용화감삽작용.
By means of UV and fluorescence spectra,the binding ratios between Dy(Ⅲ)(Trp)_3 and DNA.in physiological pH environment(pH7.40)were determined as n_(Dy(Ⅲ)(Trp)_3)：n_(DNA)=2：1 and n_(Dy(Ⅲ)：n_(Trp)=1:3.The binding constant of K_(25℃)=5.75 x10~7 L·mol~-1 and K_(37℃)=3.27×10~5 L·mol~(-1) were obtained by double reciprocal method.Thermodynamic function computation demonstrates that △_rS_m~⊕：and △_rH_m~☉：are the primary driving power of the interaction between Dy(Ⅲ)(Trp)_3 and DNA.By combination analysis of the Scatchard and viscometry methods,we suggested that the interaction mode between Dy(Ⅲ)(Trp)s complex and herring sperm DNA be electrostatic and intercalation bindings.