检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2010年
2期
113-117
,共5页
穿通支原体%肿瘤%组织浸润%淋巴结转移
穿通支原體%腫瘤%組織浸潤%淋巴結轉移
천통지원체%종류%조직침윤%림파결전이
Mycoplasma penetrans%Tumor%Tissue infiltration%Lymphatic metastasis
目的 探讨穿通支原体(Mpe)感染对肿瘤浸润和转移的影响.方法 收集53例膀胱移行细胞癌(TCCB)和40例直肠癌患者的手术切除组织标本和血液标本;以34例前列腺炎、膀胱炎及前列腺增生患者,21例直肠息肉患者的组织标本和血液标本及44名健康体检者血液标本作为对照.192例研究对象(共340份标本)分别进行Mpe分离培养鉴定,可疑阳性及阳性菌株用聚合酶链反应(PCR)予以核实.结果 TCCB组Mpe累积分离率(43.4%,23/53)与前列腺炎组(17.6%,6/34)比较,差异有统计学意义(P<0.05);直肠癌组Mpe累积分离率(70.0%,28/40)与直肠息肉组 (4.8%,1/21)比较,差异有统计学意义 (P<0.01);肿瘤组Mpe总累积分离率 (54.8%,51/93)与健康体检组 (0.0%,0/44 )比较,差异有统计学意义(P<0.01);Mpe 阳性TCCB组组织浸润检出率与Mpe阴性组比较,差异有统计学意义(P<0.05);Mpe阳性直肠癌组组织浸润检出率与Mpe阴性组比较,差异有统计学意义(P<0.05);Mpe阳性直肠癌组淋巴结转移检出率与Mpe阴性组比较,差异有统计学意义(P<0.05).结论 穿通支原体感染与肿瘤组织浸润以及转移相关联.
目的 探討穿通支原體(Mpe)感染對腫瘤浸潤和轉移的影響.方法 收集53例膀胱移行細胞癌(TCCB)和40例直腸癌患者的手術切除組織標本和血液標本;以34例前列腺炎、膀胱炎及前列腺增生患者,21例直腸息肉患者的組織標本和血液標本及44名健康體檢者血液標本作為對照.192例研究對象(共340份標本)分彆進行Mpe分離培養鑒定,可疑暘性及暘性菌株用聚閤酶鏈反應(PCR)予以覈實.結果 TCCB組Mpe纍積分離率(43.4%,23/53)與前列腺炎組(17.6%,6/34)比較,差異有統計學意義(P<0.05);直腸癌組Mpe纍積分離率(70.0%,28/40)與直腸息肉組 (4.8%,1/21)比較,差異有統計學意義 (P<0.01);腫瘤組Mpe總纍積分離率 (54.8%,51/93)與健康體檢組 (0.0%,0/44 )比較,差異有統計學意義(P<0.01);Mpe 暘性TCCB組組織浸潤檢齣率與Mpe陰性組比較,差異有統計學意義(P<0.05);Mpe暘性直腸癌組組織浸潤檢齣率與Mpe陰性組比較,差異有統計學意義(P<0.05);Mpe暘性直腸癌組淋巴結轉移檢齣率與Mpe陰性組比較,差異有統計學意義(P<0.05).結論 穿通支原體感染與腫瘤組織浸潤以及轉移相關聯.
목적 탐토천통지원체(Mpe)감염대종류침윤화전이적영향.방법 수집53례방광이행세포암(TCCB)화40례직장암환자적수술절제조직표본화혈액표본;이34례전렬선염、방광염급전렬선증생환자,21례직장식육환자적조직표본화혈액표본급44명건강체검자혈액표본작위대조.192례연구대상(공340빈표본)분별진행Mpe분리배양감정,가의양성급양성균주용취합매련반응(PCR)여이핵실.결과 TCCB조Mpe루적분리솔(43.4%,23/53)여전렬선염조(17.6%,6/34)비교,차이유통계학의의(P<0.05);직장암조Mpe루적분리솔(70.0%,28/40)여직장식육조 (4.8%,1/21)비교,차이유통계학의의 (P<0.01);종류조Mpe총루적분리솔 (54.8%,51/93)여건강체검조 (0.0%,0/44 )비교,차이유통계학의의(P<0.01);Mpe 양성TCCB조조직침윤검출솔여Mpe음성조비교,차이유통계학의의(P<0.05);Mpe양성직장암조조직침윤검출솔여Mpe음성조비교,차이유통계학의의(P<0.05);Mpe양성직장암조림파결전이검출솔여Mpe음성조비교,차이유통계학의의(P<0.05).결론 천통지원체감염여종류조직침윤이급전이상관련.
Objective To investigate the infection of Mycoplasma penetrans(Mpe) in patients with transitional cell carcinoma of bladder (TCCB) and patients with rectal carcinoma, and find out their relationship with tissue infiltration and metastasis of the tumor. Methods Blood and tissue specimens were collected from 93 carcinoma patients, including 53 cases of TCCB and 40 cases of rectal carcinoma. Meanwhile, there were 34 patients with prostatitis, cystitis and hyperplasia of prostate gland and 21patients with proctopolypus and 44 healthy subjects were performed as controls. The Mpe were isolated and cultured from these samples (total: 192 cases and 340 specimens) and the positive and suspected-positive isolates were confirmed by nested polymerase chain reaction(nPCR). Results The rate of Mpe in TCCB group(43.4%,23/53)was significantly higher than that in the prostatitis group(17.6%, 6/34, P<0.05), and the rate in the rectal carcinoma group(70.0%,28/40)was also significantly higher than that in the proctopolypus group(4.8%, 1/21, P<0.01). The total positive rate of Mpe (54.8%,51/93) in tumor cases was significantly higher than that in the control group (0.0%, 0/44, P<0.01). The detected rate of tissue infiltration in the Mpe infection group was significantly higher than that in non-infection group (P<0.05). The detected rate of lymphatic metastasis of rectal carcinoma patients in Mpe infection group was significantly higher than that in non-infection group (P<0.05). Conclusions The result suggests that the tissue infiltration and metastasis of TCCB and rectal carcinoma are correlated to Mpe infection.
