西安建筑科技大学学报(自然科学版)
西安建築科技大學學報(自然科學版)
서안건축과기대학학보(자연과학판)
JOURNAL OF XI'AN UNIVERSITY OF ARCHITECTURE & TECHNOLOGY
2010年
1期
77-82
,共6页
王蕾%聂麦茜%苏君梅%王志盈%吴蔓莉%杨学福
王蕾%聶麥茜%囌君梅%王誌盈%吳蔓莉%楊學福
왕뢰%섭맥천%소군매%왕지영%오만리%양학복
蒽(或菲)%原始态代谢物%生物降解%动力学%影响作用
蒽(或菲)%原始態代謝物%生物降解%動力學%影響作用
은(혹비)%원시태대사물%생물강해%동역학%영향작용
anthracene (phenanthrene)%natural metabolic mixtures%biodegradation%kinetics%effect
分别研究了芽胞杆菌CN2降解蒽产生的代谢物对蒽生物降解的影响和菲产生的代谢物对菲生物降解的影响.试验结果表明:(1)外加蒽(或菲)代谢物,对蒽(或菲)的降解均表现出促进作用.蒽156 h累计去除率可提高3.07%~6.10%,菲60 h累计去除率可提高13.14%~30.35%.(2)在芽胞杆菌CN2作用下,蒽(或菲)生物降解过程能够用一级动力学方程式较准确地描述,外加代谢物可以增大降解反应表观速率常数k值;从不同时段内蒽(或菲)的降解速率看,反应初期0~2 h内,外加代谢物对蒽(或菲)的降解速率提高最为明显,对蒽可提高0.281~1.20 mg/L·h,对菲可提高0.570~1.13 mg/L·h.(3)外加代谢物对蒽(或菲)降解液的pH值有缓冲作用,使降解液的pH值随降解时间下降趋势减缓.
分彆研究瞭芽胞桿菌CN2降解蒽產生的代謝物對蒽生物降解的影響和菲產生的代謝物對菲生物降解的影響.試驗結果錶明:(1)外加蒽(或菲)代謝物,對蒽(或菲)的降解均錶現齣促進作用.蒽156 h纍計去除率可提高3.07%~6.10%,菲60 h纍計去除率可提高13.14%~30.35%.(2)在芽胞桿菌CN2作用下,蒽(或菲)生物降解過程能夠用一級動力學方程式較準確地描述,外加代謝物可以增大降解反應錶觀速率常數k值;從不同時段內蒽(或菲)的降解速率看,反應初期0~2 h內,外加代謝物對蒽(或菲)的降解速率提高最為明顯,對蒽可提高0.281~1.20 mg/L·h,對菲可提高0.570~1.13 mg/L·h.(3)外加代謝物對蒽(或菲)降解液的pH值有緩遲作用,使降解液的pH值隨降解時間下降趨勢減緩.
분별연구료아포간균CN2강해은산생적대사물대은생물강해적영향화비산생적대사물대비생물강해적영향.시험결과표명:(1)외가은(혹비)대사물,대은(혹비)적강해균표현출촉진작용.은156 h루계거제솔가제고3.07%~6.10%,비60 h루계거제솔가제고13.14%~30.35%.(2)재아포간균CN2작용하,은(혹비)생물강해과정능구용일급동역학방정식교준학지묘술,외가대사물가이증대강해반응표관속솔상수k치;종불동시단내은(혹비)적강해속솔간,반응초기0~2 h내,외가대사물대은(혹비)적강해속솔제고최위명현,대은가제고0.281~1.20 mg/L·h,대비가제고0.570~1.13 mg/L·h.(3)외가대사물대은(혹비)강해액적pH치유완충작용,사강해액적pH치수강해시간하강추세감완.
The effects of metabolites of anthracene or phenanthrene on the biodegradation of anthracene or phenanthrene have been studied. The results showed that the natural state of metabolic mixtures of anthracene or phenanthrene exhibited promoting effects on biodegradation of anthracene or phenanthrene, the removing rates of anthracene were increased by 3 07%~6.10% within 156 h, and those of phenanthrene were 13.14%~30.35% within 60h. The biodegradation processes of anthracene or phenanthrene by Bacillus sp. CN2 could be described as first-order kinetics, and the only difference was that the values of k (the rate constant in the kinetic equation) for control groups were less than that of the degradation systems with addition of metabolic mixtures. The results also indicated that adding metabolic mixtures could enhance the biodegradation rate of anthracene and phenanthrene more obvious during earlier degradation stages than later stages, and for anthracene, the degradation rate increased by 0.281~1.20 mg/L·h within 2 h,and for phenanthrene, it was 0.570~1.13 mg/L·h. The metabolic mixtures could function as pH value buffer actions for the degradation fluids, so the pH values decreased slower than control groups.