CAJ | 학술논문

目的探讨粒细胞集落刺激因子(G-CSF)在缺血脑保护中的作用机制.方法制作大鼠大脑中动脉栓塞模型,7 d后断头取脑做冰冻切片,用免疫荧光双标的方法观察缺血周边区与假手术组相同部位G-CSF受体、胶质细胞源性神经营养因子(GDNF)和微管相关蛋白2(MAP2)、神经胶质原酸性蛋白(GFAP)的共表达情况.结果 G-CSF受体和GDNF在正常大鼠脑内广泛表达于神经元,不表达于星形胶质细胞;但在缺血周边区,星形胶质细胞亦部分表达G-CSF受体和GDNF.在正常脑组织,大部分G-CSF受体阳性的细胞也表达GDNF.结论脑缺血可诱导缺血周边区星形胶质细胞表达G-CSF受体和GDNF,推测缺血后的内源性神经保护作用可能与缺血周边区星形胶质细胞的G-CSF受体表达以及GDNF产生有关.
목적 탐토립세포집락자격인자(G-CSF)재결혈뇌보호중적작용궤제.방법 제작대서대뇌중동맥전새모형,7 d후단두취뇌주빙동절편,용면역형광쌍표적방법관찰결혈주변구여가수술조상동부위G-CSF수체、효질세포원성신경영양인자(GDNF)화미관상관단백2(MAP2)、신경효질원산성단백(GFAP)적공표체정황.결과 G-CSF수체화GDNF재정상대서뇌내엄범표체우신경원,불표체우성형효질세포;단재결혈주변구,성형효질세포역부분표체G-CSF수체화GDNF.재정상뇌조직,대부분G-CSF수체양성적세포야표체GDNF.결론 뇌결혈가유도결혈주변구성형효질세포표체G-CSF수체화GDNF,추측결혈후적내원성신경보호작용가능여결혈주변구성형효질세포적G-CSF수체표체이급GDNF산생유관.
Objective To explore the neuroprotective mechanisms of granulocyte colony stimulating factor (G-CSF) in ischemic brain injury. Methods Brain tissues were taken out from MCAO and sham operated Sprague-Dawley rats 7 days after operation. The expression of G-CSFR, GDNF, MAP2 and GFAP was measured by using immunofluorescence co-staining. Results The expression of G-CSFR and GDNF were widely distributed in the neurons in normal brain tissues, not in the astrocytes. However, in ischemic peripheral zone, part of G-CSFR and GDNF positive cells merged with GFAP positive cells, in normal brain tissues, most G-CSFR positive cells were co-expressed with GDNF. Conclusion Cerebral ischemia induces astrocytes to express G-CSFR and GDNF, suggesting that endogenous neuroprotection by cerebral ischemia may be related with the expression and production of G-CSFR and GDNF in astrocytes in ischemic peripheral zone.