CAJ | 학술논문

目的观察骨巨细胞瘤组织中人端粒酶逆转录酶(hTERT)基因的表达,并探讨其临床意义.方法选择我院2008年1月至2011年12月病理科保存的标本,标本中骨巨细胞瘤84例、骨质增生骨组织32例、异位骨化组织28例,对照组为正常骨组织20例；采用原位分子杂交技术检测和定位标本中的端粒酶hTERT基因,并用图像分析系统分析其表达水平.、结果端粒酶hTERT基因在骨巨细胞瘤组织中的表达阳性率为46.4％(39/84),表达强度与骨巨细胞瘤的分化程度明显相关(P＜0.05),表达水平与肿瘤细胞的分布定位一致；端粒酶hTERT基因在骨质增生、异位骨化和正常骨组织中的表达均为阴性.结论骨巨细胞瘤组织的恶化可能与端粒酶hTERT基因相关.
목적 관찰골거세포류조직중인단립매역전록매(hTERT)기인적표체,병탐토기림상의의.방법 선택아원2008년1월지2011년12월병이과보존적표본,표본중골거세포류84례、골질증생골조직32례、이위골화조직28례,대조조위정상골조직20례；채용원위분자잡교기술검측화정위표본중적단립매hTERT기인,병용도상분석계통분석기표체수평.、결과 단립매hTERT기인재골거세포류조직중적표체양성솔위46.4％(39/84),표체강도여골거세포류적분화정도명현상관(P＜0.05),표체수평여종류세포적분포정위일치；단립매hTERT기인재골질증생、이위골화화정상골조직중적표체균위음성.결론 골거세포류조직적악화가능여단립매hTERT기인상관.
Objective To study the clinical significance of human telomerase reverse transcriptase (hTERT) gene expression in giant cell tumor of bone.Methods By using in situ hybridization (ISH) techniques,the clinical significance of hTERT mRNA expression was tested in 84 cases of giant cell tumor of bone,32 cases of hyperostosis,28 cases of heterotopic ossification and 20 cases of normal bone tissues.These cases were collected from Jan.2008 to Dec.2011 in our department.The expression level and the location of hTERT gene were observed by using the image analyzing system.Results hTERT was expressed in 46.4％ (39/84) of giant cell tumors of bone and it had an obvious correlation with the differentiation level of tumor cells ( P ＜ 0.05 ).There was a close correlation between the expression of hTERT and the location of tumor cells.hTERT gene was not detected in 32 cases of hyperostosis,28 cases of heterotopic ossification or 20 cases of normal bone tissues.Conclusion hTERT gene may play an important role in the pathogenesis of giant cell tumors of bone.