CAJ | 학술논문

为了研究中枢神经系统是否表达促血小板生成素(TPO)的受体c-mpl和TPO对神经细胞生长和保护作用,用免疫组织化学和RT-PCR方法分析c-mpl表达,用MTT,annexinV方法和流式细胞术检测、观察TPO对神经细胞生长和抗凋亡作用.结果表明,人中枢神经系统和小鼠神经细胞均表达TPO受体c-mpl,人大脑半球和小脑及小鼠神经干细胞株C17.2在mRNA水平表达c-mpl,进一步在蛋白水平证实TPO受体c-mpl在人大脑半球神经细胞表达,TPO对C17.2细胞有生长促进作用和抗凋亡作用.结论:本研究首次证实中枢神经系统表达TPO受体c-mpl和TPO有促进体外神经细胞生长和抗凋亡的作用.
위료연구중추신경계통시부표체촉혈소판생성소(TPO)적수체c-mpl화TPO대신경세포생장화보호작용,용면역조직화학화RT-PCR방법분석c-mpl표체,용MTT,annexinV방법화류식세포술검측、관찰TPO대신경세포생장화항조망작용.결과표명,인중추신경계통화소서신경세포균표체TPO수체c-mpl,인대뇌반구화소뇌급소서신경간세포주C17.2재mRNA수평표체c-mpl,진일보재단백수평증실TPO수체c-mpl재인대뇌반구신경세포표체,TPO대C17.2세포유생장촉진작용화항조망작용.결론:본연구수차증실중추신경계통표체TPO수체c-mpl화TPO유촉진체외신경세포생장화항조망적작용.
To identify the expression of thrombopoietin (TPO) receptors (c-mpl) on central nervous system (CNS)and to evaluate the role of TPO on neural cell proliferation and protection, immunohistochemical staining, RT-PCR,MTT, and annexin-V methods were used in this study. The results showed the expression of TPO receptor on human CNS and murine neural cells. C-mpl mRNA was identified in human cerebral hemispheres and cerebellum, and mouse neural cell line C17.2 by RT-PCR. C-mpl was also confirmed in human cerebral hemispheres by irmmunohistostaining with con-focal microscopy. Furthermore, TPO had a stimulating effect on the growth of in vitro neural cell C17.2 by MTT assay. The anti-apoptotic effect of TPO on C17.2 cells was also demonstrated by staining with annexin-V and PI.In conclusion, the first evidence showed the expression of TPO receptor c-mpl in central nervous system. Moreover, the effect of TPO on neural cell proliferation and anti-apoptosis was also demonstrated on in vitro neural cells.