CAJ | 학술논문

目的:探讨乳腺癌组织中Survivin表达的临床病理学意义及其与癌细胞增殖的关系.方法:应用免疫组织化学SP法检测Survivin、Ki-67蛋白在96例乳腺癌组织中及20例正常乳腺组织的表达,分析Survivin 表达与Ki-67增殖指数及各临床病理因素的关系.结果:Survivin阳性表达乳腺癌的Ki-67增殖指数(35.32±21.28%)明显高于Survivin阴性者(20.42±11.34%),Survivin表达与肿瘤细胞增殖呈正相关(P<0.01);Survivin在乳腺癌组织中的表达率为70.83%(68/96),正常乳腺组织未见Survivin表达;Survivin蛋白的表达与临床分期、淋巴结转移和5年生存率有关(P<0.05),与年龄、是否绝经、肿瘤大小和组织学分级均无关.结论: Survivin不仅参与凋亡的调控,还促进细胞增殖. Survivin蛋白在乳腺癌中表达上调,提示其通过抑制凋亡在乳腺癌发生、发展中起重要作用,过度表达提示预后不良.
목적:탐토유선암조직중Survivin표체적림상병이학의의급기여암세포증식적관계.방법:응용면역조직화학SP법검측Survivin、Ki-67단백재96례유선암조직중급20례정상유선조직적표체,분석Survivin 표체여Ki-67증식지수급각림상병리인소적관계.결과:Survivin양성표체유선암적Ki-67증식지수(35.32±21.28%)명현고우Survivin음성자(20.42±11.34%),Survivin표체여종류세포증식정정상관(P<0.01);Survivin재유선암조직중적표체솔위70.83%(68/96),정상유선조직미견Survivin표체;Survivin단백적표체여림상분기、림파결전이화5년생존솔유관(P<0.05),여년령、시부절경、종류대소화조직학분급균무관.결론: Survivin불부삼여조망적조공,환촉진세포증식. Survivin단백재유선암중표체상조,제시기통과억제조망재유선암발생、발전중기중요작용,과도표체제시예후불량.
Objective:To study the expression of inhibitor apoptosis protein survivin in breast carcimoma and its correlation with clinicopathological feature and cell proliferation. Methods: Using streptavidin-biotin peroxidase(SP) method of immunohistochemistry,expressions of survivin and Ki67 of 20 cases of normal tissuse of mammary and 96 cases of breast carcinoma tissues were detected.The relationship between the Survivin expression and proliferation index,or the clinicopathological parameters was analyzed. Results: The proliferation index in Survivin-positive breast carcinoma(35.32±21.28%) was significantly higher than that in Survivin-negative breast carcinoma(20.42±11.34%)(P<0.05),positive correlation between survivin expression and proliferative activity of tumor cell measured by Ki-67 index was found.The positive rate of Survivin in breast carcinoma was 70.83%(61/96),no expression of Survivin was detected in normal mammary tissues.Surivivin expression was related to clinical stage and lymph node metastasis and to 5 year survival rate (P<0.05),but not to age,menopause,tumor size and histological grade. Conclusion: Survivin is not only involved in control of apoptosis,but also accelerate proliferation.Survivin gene may play an important role in tumorigenesis and progression of breast carcinoma and its overexpression indicates worse prognosis.