华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2009年
6期
775-778
,共4页
刘群%陈雯%朱桂金%王昕荣%胡娟%魏玉兰%任新玲%章汉旺%岳静
劉群%陳雯%硃桂金%王昕榮%鬍娟%魏玉蘭%任新玲%章漢旺%嶽靜
류군%진문%주계금%왕흔영%호연%위옥란%임신령%장한왕%악정
体外授精%卵裂期胚胎%细胞凋亡%胚胎形态
體外授精%卵裂期胚胎%細胞凋亡%胚胎形態
체외수정%란렬기배태%세포조망%배태형태
in vitro fertilization%cleavage stage embryo%apoptosis%embryo morphology
目的 评估体外授精的人卵裂期胚胎中是否存在凋亡,探讨卵裂期胚胎的凋亡征象与胚胎形态之间的关系.方法 结合末端脱氧核苷酸转移酶介导dUTP缺口末端标记法(TUNEL)原位检测DNA凋亡和测定Caspase 3的表达水平,检测人卵裂期胚胎中的凋亡.并分析卵裂期胚胎中胞质碎片占胚胎总体积的比例和发育速度与TUNEL阳性率和Caspase 3的表达强度之间的关系.结果 碎片比>20%组胚胎的凋亡率明显高于碎片比≤20%的胚胎(P<0.01),Caspase 3平均荧光强度在碎片比>50%组明显升高(P<0.05).发育正常、迟缓和停滞胚胎之间胚胎凋亡率差异无显著性意义.发育停滞组的Caspase 3平均荧光强度高于发育正常和迟缓组(P<0.05).结论 人体外授精的卵裂期胚胎中存在凋亡征象,凋亡主要与胞质碎片有关,胚胎发育停滞可能是胚胎凋亡的早期表现.
目的 評估體外授精的人卵裂期胚胎中是否存在凋亡,探討卵裂期胚胎的凋亡徵象與胚胎形態之間的關繫.方法 結閤末耑脫氧覈苷痠轉移酶介導dUTP缺口末耑標記法(TUNEL)原位檢測DNA凋亡和測定Caspase 3的錶達水平,檢測人卵裂期胚胎中的凋亡.併分析卵裂期胚胎中胞質碎片佔胚胎總體積的比例和髮育速度與TUNEL暘性率和Caspase 3的錶達彊度之間的關繫.結果 碎片比>20%組胚胎的凋亡率明顯高于碎片比≤20%的胚胎(P<0.01),Caspase 3平均熒光彊度在碎片比>50%組明顯升高(P<0.05).髮育正常、遲緩和停滯胚胎之間胚胎凋亡率差異無顯著性意義.髮育停滯組的Caspase 3平均熒光彊度高于髮育正常和遲緩組(P<0.05).結論 人體外授精的卵裂期胚胎中存在凋亡徵象,凋亡主要與胞質碎片有關,胚胎髮育停滯可能是胚胎凋亡的早期錶現.
목적 평고체외수정적인란렬기배태중시부존재조망,탐토란렬기배태적조망정상여배태형태지간적관계.방법 결합말단탈양핵감산전이매개도dUTP결구말단표기법(TUNEL)원위검측DNA조망화측정Caspase 3적표체수평,검측인란렬기배태중적조망.병분석란렬기배태중포질쇄편점배태총체적적비례화발육속도여TUNEL양성솔화Caspase 3적표체강도지간적관계.결과 쇄편비>20%조배태적조망솔명현고우쇄편비≤20%적배태(P<0.01),Caspase 3평균형광강도재쇄편비>50%조명현승고(P<0.05).발육정상、지완화정체배태지간배태조망솔차이무현저성의의.발육정체조적Caspase 3평균형광강도고우발육정상화지완조(P<0.05).결론 인체외수정적란렬기배태중존재조망정상,조망주요여포질쇄편유관,배태발육정체가능시배태조망적조기표현.
Objective To evaluate whether apoptosis exists in human cleavage stage embryos from in vitro fertilization and the relationship between apoptosis in cleavage stage embryos and morphological characteristics on D3.Methods Apoptosis of human cleavage stage embryos and the expression of Caspase 3 were detected by using the terminal transferase-mediated DNA end labeling(TUNEL) assay.The TUNEL-positive rate and the average fluorescent intensity were determined according to the fragmentation rate and developmental rate.Results Apoptosis rate in embryos with>20% fragmentation was significantly higher than that in other groups(P<0.01).The average fluorescent intensity was significantly increased in embryos with>50% fragmentation(P<0.05).There was no significant difference in apoptosis rate among the normal developmental embryos,slow developmental embryos and arrested embryos.The average fluorescent intensity in arrested embryos was strong than that in the normal developmental embryos and slow developmental embryos(P<0.05).Conclusion Apoptosis does exist in cleavage stage embryos from in vitro fertilization.Apoptosis in cleavage stage embryo is strongly correlated with cellular fragments.The early developmental arrest of the human preimplantation embryos may be the early phenomenon of apoptosis.
