CAJ | 학술논문

利用前期研究建立的一套水稻品种DNA指纹检测技术体系,对2002-2006年浙江省主要的98个水稻品种、以及2007-2008年155个浙江省区试水稻品种(181个次,含26个续试品种)进行了DNA指纹检测,构建了279个次水稻品种×12个微卫星标记的DNA指纹图谱数据库.系统分析了指纹库中各标记的多态性和品种的相似性.基于构建的指纹数据库,对所有品种的特异性及区试续试品种年度间的一致性进行了鉴定.结果发现5组11个品种组内品种间特异性不明显,1个续试品种年度间鉴定结果不一致.
이용전기연구건립적일투수도품충DNA지문검측기술체계,대2002-2006년절강성주요적98개수도품충、이급2007-2008년155개절강성구시수도품충(181개차,함26개속시품충)진행료DNA지문검측,구건료279개차수도품충×12개미위성표기적DNA지문도보수거고.계통분석료지문고중각표기적다태성화품충적상사성.기우구건적지문수거고,대소유품충적특이성급구시속시품충년도간적일치성진행료감정.결과발현5조11개품충조내품충간특이성불명현,1개속시품충년도간감정결과불일치.
A total of 98 major rice varieties in Zhejiang Province from 2002 to 2006 and 155 rice varieties in Zhejiang provincial regional trial from 2007 to 2008 were assayed with a set of DNA fingerprint testing system on rice variety. A DNA fingerprint database containing genotypic data of 279 rice variety times 12 marker loci was constructed. The polymorphism of different markers and genetic similarity of varieties in the DNA fingerprint database were analyzed. Based on the DNA fingerprint database,the distinctness of all rice varieties and consistency of the same varieties in different years were identified. It was found that Ⅱ varieties of 5 groups had no obvious distinctness within groups ,and discrepancy existed in the same variety in different years.