CAJ | 학술논문

背景与目的:氧化苦参碱(oxymatrine,OM)是中药苦参的主要有效成分,具有抗纤维化、抗病毒等作用,对放疗和化疗后白细胞减少亦有一定提升作用,研究显示氧化苦参碱能一定程度杀伤肿瘤细胞,抑制肿瘤细胞的侵袭,并可作为辅助抗癌药物用于临床.本研究旨在通过观察氧化苦参碱对人胃癌SGC-7901细胞增殖和血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,探讨氧化苦参碱抗肿瘤的作用机制.方法:体外培养入胃癌SGC-7901细胞,采用MTT法观察不同浓度和作用时间下氧化苦参碱对SGC-7901细胞的抑制情况;采用免疫组织化学法检测肿瘤细胞内VEGF蛋白的表达;RT-PCR法检测氧化苦参碱作用下SGC-7901细胞中VEGF mRNA的转录情况.结果:低质量浓度氧化苦参碱(0.5 mg/mL)对SGC-7901细胞增殖抑制作用不明显(P>0.05),但当其质量浓度达到1 mg/mL以上时,则能显著抑制细胞的增殖,抑制效应随着时间和浓度的增加呈逐渐增强,同时伴有癌细胞内VEGFmRNA转录和蛋白表达的降低(P<0.05). 结论:氧化苦参碱在体外能显著抑制SGC-7901细胞增殖,并能抑制VEGF基凶的转录和表达,提示氧化苦参碱有抑制肿瘤血管生成的潜在作用.
배경여목적:양화고삼감(oxymatrine,OM)시중약고삼적주요유효성분,구유항섬유화、항병독등작용,대방료화화료후백세포감소역유일정제승작용,연구현시양화고삼감능일정정도살상종류세포,억제종류세포적침습,병가작위보조항암약물용우림상.본연구지재통과관찰양화고삼감대인위암SGC-7901세포증식화혈관내피생장인자(vascular endothelial growth factor,VEGF)표체적영향,탐토양화고삼감항종류적작용궤제.방법:체외배양입위암SGC-7901세포,채용MTT법관찰불동농도화작용시간하양화고삼감대SGC-7901세포적억제정황;채용면역조직화학법검측종류세포내VEGF단백적표체;RT-PCR법검측양화고삼감작용하SGC-7901세포중VEGF mRNA적전록정황.결과:저질량농도양화고삼감(0.5 mg/mL)대SGC-7901세포증식억제작용불명현(P>0.05),단당기질량농도체도1 mg/mL이상시,칙능현저억제세포적증식,억제효응수착시간화농도적증가정축점증강,동시반유암세포내VEGFmRNA전록화단백표체적강저(P<0.05). 결론:양화고삼감재체외능현저억제SGC-7901세포증식,병능억제VEGF기흉적전록화표체,제시양화고삼감유억제종류혈관생성적잠재작용.
Background and purpose: Oxymatrine, which is the main effective component of Sopkora flavescens Ait, has anti-fibrosis and antiviral activities, and also has a good effect on leukopenia after either chemotherapy or radiotherapy. Recent studies showed that oxymatrine has the abilities of anti-invasion and killing tumor cells in some degree, and as a supplementary anticancer drug in chemical therapy. In this study, we investigated the antitumor mechanism of oxymatrine by observing cell proliferation and VEGF expression in human gastric cancer SGC-7901 cell line. Methods: Human gastric cancer SGC-7901 cells was cultured in vitro and treated with oxymatrine, then cell proliferation was examined by the method of MTT. Immunohistochemistry was applied to examine the protein expression of VEGE The transcriptions of VEGF mRNA were demonstrated by RT-PCR technique.Results: Low-dose (0.5 mg/mL) oxymatrine has a mild inhibitory effect on cellular proliferation of SGC-7901 cells (P>0.05). When concentration exceeded 1 mg/mL, oxymatrine significantly inhibited cellular proliferation in a time-and concentration-dependent manner, and down-regulated the mRNA and protein expression of VEGF (P<0.05).Conclusion: Within a certain drug concentration, oxymatrine can inhibit the proliferation of SGC-7901 cells and play a potential role in inhibiting angiogenesis by down-regulating the expression of VEGF.