CAJ | 학술논문

利用PCR-RFLP方法对3个地理居群泥蚶的核糖体DNA ITS区进行分析,从分子水平探讨三者的遗传多样性及亲缘关系.结果表明:在选用的6种限制性内切酶中,BstHH I、Hae III和Sse9 I为多态性的限制性内切酶,Msp I、Rsa I和Taq I为单态性限制性内切酶.对酶切图谱进行数理统计后发现宁波海区泥蚶群体内核苷酸多样性指数π值为0.001 7,温州海区泥蚶π值为0.001 6,而莆田海区泥蚶π值为0.001 1,说明宁波海区泥蚶群体内遗传多样性最大,温州海区群体内遗传多样性次之,莆田海区群体内遗传多样性最小.对所有酶切图谱的复合单倍型进行统计,共发现10种复合单倍型,其中宁波海区泥蚶群体内所含的复合单倍型类型最多为7种,其他2个海区均为6种,进一步证实宁波海区泥蚶群体内遗传多样性最大.另外,通过比较发现每个地理居群都有其特有的复合单倍型,如ABBABA型和BAABAA型复合单倍型是宁波海区泥蚶样本特有的,AAABBA型复合单倍型是莆田海区泥蚶样本特有的,温州海区泥蚶样本则具有AAACAA、BCAABA 2种特有的复合单倍型.依据3个地理居群泥蚶间的净遗传距离Pnet值及聚类分析结果可知,莆田与温州海区泥蚶间亲缘关系最远(Pnet=0.000 8),宁波与温州海区泥蚶间亲缘关系最近(Pnet=0.000 3),宁波与莆田海区泥蚶间亲缘关系介于以上二者之间(Pnet=0.000 4).
이용PCR-RFLP방법대3개지리거군니감적핵당체DNA ITS구진행분석,종분자수평탐토삼자적유전다양성급친연관계.결과표명:재선용적6충한제성내절매중,BstHH I、Hae III화Sse9 I위다태성적한제성내절매,Msp I、Rsa I화Taq I위단태성한제성내절매.대매절도보진행수리통계후발현저파해구니감군체내핵감산다양성지수π치위0.001 7,온주해구니감π치위0.001 6,이보전해구니감π치위0.001 1,설명저파해구니감군체내유전다양성최대,온주해구군체내유전다양성차지,보전해구군체내유전다양성최소.대소유매절도보적복합단배형진행통계,공발현10충복합단배형,기중저파해구니감군체내소함적복합단배형류형최다위7충,기타2개해구균위6충,진일보증실저파해구니감군체내유전다양성최대.령외,통과비교발현매개지리거군도유기특유적복합단배형,여ABBABA형화BAABAA형복합단배형시저파해구니감양본특유적,AAABBA형복합단배형시보전해구니감양본특유적,온주해구니감양본칙구유AAACAA、BCAABA 2충특유적복합단배형.의거3개지리거군니감간적정유전거리Pnet치급취류분석결과가지,보전여온주해구니감간친연관계최원(Pnet=0.000 8),저파여온주해구니감간친연관계최근(Pnet=0.000 3),저파여보전해구니감간친연관계개우이상이자지간(Pnet=0.000 4).
RFLP analysis was used to investigate the genetic diversity and phylogenetic relationship of three populations of Tegillarca granosa based on the ribosomal internal transcribed spacer (ITS) region. The results showed that BstHH I, Hae III and Sse9 I were polymorphic restriction endonucleases in PCR product digestion, while Msp I, Rsa I and Taq I were monomorphic. Nucleotide diversity index (π value) of population in Ningbo was 0.001 7, and π values in Wenzhou and Fujian were 0.001 2 and 0.001 1, respectively. It was shown that the genetic diversity was the highest in Ningbo population followed by Wenzhou population and Ningbo population. According to the Pnet value among three populations and cluster analysis results, it was concluded that the phylogenetic relationship between Fujian population and Wenzhou population was farther than that of Fujian population and Ningbo population and the geography relationship of Wenzhou population and Ningbo population was the closest.