CAJ | 학술논문

人胰高血糖素样肽1（GLP1）是一种短肽激素，对Ⅱ型糖尿病具有很好的疗效。本研究在设计合成GLP1基因并构建植物表达载体的基础上，通过农杆菌介导将GLP1基因导入番茄基因组中，经过PCR扩增和Southern Blot分析，证实GLP1基因已整合进入9个株系的番茄基因组中。Western Blot检测表明，其中4个株系转基因番茄的叶片能够检测到hGLP1融合蛋白的表达。通过Ni—NTA亲和层析分离纯化转基因番茄表达的hGLP1融合蛋白，动物实验表明该融合蛋白具有显著的降血糖生物活性。本研究结果将为转基因番茄作为生物反应器表达药用蛋白提供重要的理论和技术支持，并将为培育具有糖尿病治疗功能的番茄新品种奠定基础。
인이고혈당소양태1（GLP1）시일충단태격소，대Ⅱ형당뇨병구유흔호적료효。본연구재설계합성GLP1기인병구건식물표체재체적기출상，통과농간균개도장GLP1기인도입번가기인조중，경과PCR확증화Southern Blot분석，증실GLP1기인이정합진입9개주계적번가기인조중。Western Blot검측표명，기중4개주계전기인번가적협편능구검측도hGLP1융합단백적표체。통과Ni—NTA친화층석분리순화전기인번가표체적hGLP1융합단백，동물실험표명해융합단백구유현저적강혈당생물활성。본연구결과장위전기인번가작위생물반응기표체약용단백제공중요적이론화기술지지，병장위배육구유당뇨병치료공능적번가신품충전정기출。
Human glucagon-like peptide 1 （GLP1） is a short peptide hormone with especial treatment to type 2 diabetes. This study based on design composition of GLP1 gene and construction of plant expression vector. GLP1 gene was introduced into tomato genome through Agrobacterium turafaciens mediation. PCR and Southern Blot analysis have confirmed that the GLP1 gene had been integrated into the genome of 9 regenerated tomato lines. Western Blot suggested that leaves of four transgenic tomato lines could express hGLP1 fusion protein. The hGLP1 fusion protein expressed in the transgenic tomato was isolated and purified with Ni-NTA affinity chromatography, and showed obvious biological activity in reducing blood sugar with mice experiment. This study should provide theoretical and technological support for producing pharmaceutical proteins in transgenic tomato as bioreactor, and establishes foundation for breeding new tomato varieties with treatment to diabetes.