中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
12期
1092-1094
,共3页
脑梗死%黄体酮%脑梗死体积%神经功能%大鼠
腦梗死%黃體酮%腦梗死體積%神經功能%大鼠
뇌경사%황체동%뇌경사체적%신경공능%대서
Cerebral infarction%Progesterone%Infarct volume%Neurological functions%Rat
目的 研究黄体酮对脑梗死体积及行为学的影响,评价其对脑梗死大鼠的治疗价值.方法 按随机数字表法将健康成年雄性Sprague-Dawley大鼠分为4组,即假手术组、模型组、溶剂治疗组和黄体酮治疗组.后3组采用线栓法制作大脑中动脉栓塞大鼠模型,假手术组只分离暴露血管、不结扎动脉、不插入尼龙鱼线；黄体酮治疗组造模成功后腹腔注射黄体酮,溶剂治疗组给予等体积的环糊精溶液治疗.分别于脑梗死后1d、2d、3d用Zea-Longer评分评价大鼠神经功能的缺损程度,并于脑梗死后3d通过TTC染色检测脑梗死体积.结果 Zea-Longer评分显示,造模前所有大鼠均没有神经功能缺损症状,评分为0.成模后,除假手术组外,其余大鼠均有不同程度的神经功能缺损；黄体酮治疗组大鼠[(3.00±0.63)分,(2.83±0.75)分,(2.00±0.89)分]在脑梗死后1d、2d、3d神经功能评分低于模型组[(4.00±0.89)分,(3.83±0.75)分,(3.16 ±0.75)分]及溶剂治疗组[(3.67±1.21)分,(3.50±1.05)分,(2.83±0.76)分],差异有显著性(P＜0.05).TTC染色表明,脑梗死体积在模型组[(23.74±4.48)％]与溶剂治疗组[(24.42±7.07)％]均比较大,且二者比较差异无统计学意义(P＞0.05)；经黄体酮治疗后[(15.03±3.75)％]脑梗死体积明显缩小,较模型组与溶剂治疗组差异均有显著性(P＜0.05).结论 黄体酮治疗可以显著减小脑梗死体积并明显促进脑梗死大鼠神经功能的恢复,其对脑梗死大鼠动物模型具有很好的治疗价值.
目的 研究黃體酮對腦梗死體積及行為學的影響,評價其對腦梗死大鼠的治療價值.方法 按隨機數字錶法將健康成年雄性Sprague-Dawley大鼠分為4組,即假手術組、模型組、溶劑治療組和黃體酮治療組.後3組採用線栓法製作大腦中動脈栓塞大鼠模型,假手術組隻分離暴露血管、不結扎動脈、不插入尼龍魚線；黃體酮治療組造模成功後腹腔註射黃體酮,溶劑治療組給予等體積的環糊精溶液治療.分彆于腦梗死後1d、2d、3d用Zea-Longer評分評價大鼠神經功能的缺損程度,併于腦梗死後3d通過TTC染色檢測腦梗死體積.結果 Zea-Longer評分顯示,造模前所有大鼠均沒有神經功能缺損癥狀,評分為0.成模後,除假手術組外,其餘大鼠均有不同程度的神經功能缺損；黃體酮治療組大鼠[(3.00±0.63)分,(2.83±0.75)分,(2.00±0.89)分]在腦梗死後1d、2d、3d神經功能評分低于模型組[(4.00±0.89)分,(3.83±0.75)分,(3.16 ±0.75)分]及溶劑治療組[(3.67±1.21)分,(3.50±1.05)分,(2.83±0.76)分],差異有顯著性(P＜0.05).TTC染色錶明,腦梗死體積在模型組[(23.74±4.48)％]與溶劑治療組[(24.42±7.07)％]均比較大,且二者比較差異無統計學意義(P＞0.05)；經黃體酮治療後[(15.03±3.75)％]腦梗死體積明顯縮小,較模型組與溶劑治療組差異均有顯著性(P＜0.05).結論 黃體酮治療可以顯著減小腦梗死體積併明顯促進腦梗死大鼠神經功能的恢複,其對腦梗死大鼠動物模型具有很好的治療價值.
목적 연구황체동대뇌경사체적급행위학적영향,평개기대뇌경사대서적치료개치.방법 안수궤수자표법장건강성년웅성Sprague-Dawley대서분위4조,즉가수술조、모형조、용제치료조화황체동치료조.후3조채용선전법제작대뇌중동맥전새대서모형,가수술조지분리폭로혈관、불결찰동맥、불삽입니룡어선；황체동치료조조모성공후복강주사황체동,용제치료조급여등체적적배호정용액치료.분별우뇌경사후1d、2d、3d용Zea-Longer평분평개대서신경공능적결손정도,병우뇌경사후3d통과TTC염색검측뇌경사체적.결과 Zea-Longer평분현시,조모전소유대서균몰유신경공능결손증상,평분위0.성모후,제가수술조외,기여대서균유불동정도적신경공능결손；황체동치료조대서[(3.00±0.63)분,(2.83±0.75)분,(2.00±0.89)분]재뇌경사후1d、2d、3d신경공능평분저우모형조[(4.00±0.89)분,(3.83±0.75)분,(3.16 ±0.75)분]급용제치료조[(3.67±1.21)분,(3.50±1.05)분,(2.83±0.76)분],차이유현저성(P＜0.05).TTC염색표명,뇌경사체적재모형조[(23.74±4.48)％]여용제치료조[(24.42±7.07)％]균비교대,차이자비교차이무통계학의의(P＞0.05)；경황체동치료후[(15.03±3.75)％]뇌경사체적명현축소,교모형조여용제치료조차이균유현저성(P＜0.05).결론 황체동치료가이현저감소뇌경사체적병명현촉진뇌경사대서신경공능적회복,기대뇌경사대서동물모형구유흔호적치료개치.
Objective To study the influence of progesterone (PROG) on infarct volume and functional outcome and to evaluate the therapeutic value of PROG on cerebral infarction in rats.Methods Health adult male Sprague-Dawley rats were randomly divided into sham-operated (control) group,ischemic group,vehicle-treated group and PROG-treated group.Permanent cerebral ischemia was induced by occlusion of the left middle cerebral artery (MCA) using an intraluminal filament technique.Sham-operated rats were subjected to the same surgical procedure,except that the filament was not advanced to occlude the MCA.Progesterone or 2-hydroxypropyl-β-cyclodextrin was injected intraperitoneally following permanent middle cerebral artery occlusion (PMCAO) of rats.Zea Longa test was used to evaluate their functional outcome at 1d,2d,3d after stroke.TTC staining was used to detect the infarct volume at 3d after stroke.Results The results of Zea Longa test showed that there were no functional deficits in all animals prior to ischemia.There were no significant changes in motor function in sham-operated animals across the 3 days assessment period.Both PROG and vehicle-treated rats experienced significant decline in scores following occlusion.However,PROG-treated rats (3.00 ± 0.63,2.83 ± 0.75,2.00 ± 0.89 )demonstrated a gradual improvement in scores compared with ischemic (4.00 ± 0.89,3.83 ± 0.75,3.16 ± 0.75 )and vehicle-treated rats ( 3.67 ± 1.21,3.50 ± 1.05,2.83 ± 0.76) at different times (P ＜ 0.05 ).TTC staining revealed that PROG administration significantly reduced the total infarct volume in the PROG-treated rats ( ( 15.03± 3.75) ％ ) compared with ischemic ( (23.74 ± 4.48 ) ％ ) and vehicle-treated rats ( ( 24.42 ± 7.07 ) ％,P ＜0.05).Conclusions PROG significantly reduces infarct volume and promotes the recovery of neurological functions after pMCAO,which has good therapeutic value for the rat model of cerebral infarction.
