中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2012年
9期
743-746
,共4页
任约翰%陈积贤%薛迪新%余红敏%吴伟力%张仁虎%林道浙%余铭%林肖%梁美珍
任約翰%陳積賢%薛迪新%餘紅敏%吳偉力%張仁虎%林道浙%餘銘%林肖%樑美珍
임약한%진적현%설적신%여홍민%오위력%장인호%림도절%여명%림초%량미진
胃肿瘤%微RNAs%病理学,临床%预后
胃腫瘤%微RNAs%病理學,臨床%預後
위종류%미RNAs%병이학,림상%예후
Stomach neoplasms%Micro RNAs%Pathology,clinical%Prognosis
目的 研究miR-130b和RUNX3mRNA在胃癌组织中的表达,并评价miR-130b对抑癌基因RUNX3表达的影响.方法 用荧光定量PCR技术检测40对胃癌组织及其对应的癌旁正常胃黏膜组织中miR-130b和RUNX3mRNA的表达量；用免疫组织化学SP法检测RUNX3蛋白的表达.结果 胃癌组织中miR-130b的表达显著高于对应的癌旁组织(2.18±3.75)比(2.59±3.45),P＜0.05;而胃癌组织中RUNX3mRNA的表达显著低于对应的癌旁组织(8.76±2.82)比(7.58±2.87),P ＜0.05.miR-130b和RUNX3mRNA的表达与胃癌的淋巴结转移、临床分期有关(P＜0.05),而与患者的年龄、性别、肿瘤大小、远处转移、有无浸润周边器官组织无关(均P ＞0.05).miR-130b的表达与RUNX3蛋白的核表达、细胞核+细胞质的表达存在负相关(P＜0.05),而与RUNX3mRNA、RUNX3蛋白的细胞质表达无相关性(P＞0.05).结论 miR-130b和RUNX3mRNA的异常表达与胃癌预后有关；miR-130b可能通过减少RUNX3蛋白的表达水平导致肿瘤的发生.
目的 研究miR-130b和RUNX3mRNA在胃癌組織中的錶達,併評價miR-130b對抑癌基因RUNX3錶達的影響.方法 用熒光定量PCR技術檢測40對胃癌組織及其對應的癌徬正常胃黏膜組織中miR-130b和RUNX3mRNA的錶達量；用免疫組織化學SP法檢測RUNX3蛋白的錶達.結果 胃癌組織中miR-130b的錶達顯著高于對應的癌徬組織(2.18±3.75)比(2.59±3.45),P＜0.05;而胃癌組織中RUNX3mRNA的錶達顯著低于對應的癌徬組織(8.76±2.82)比(7.58±2.87),P ＜0.05.miR-130b和RUNX3mRNA的錶達與胃癌的淋巴結轉移、臨床分期有關(P＜0.05),而與患者的年齡、性彆、腫瘤大小、遠處轉移、有無浸潤週邊器官組織無關(均P ＞0.05).miR-130b的錶達與RUNX3蛋白的覈錶達、細胞覈+細胞質的錶達存在負相關(P＜0.05),而與RUNX3mRNA、RUNX3蛋白的細胞質錶達無相關性(P＞0.05).結論 miR-130b和RUNX3mRNA的異常錶達與胃癌預後有關；miR-130b可能通過減少RUNX3蛋白的錶達水平導緻腫瘤的髮生.
목적 연구miR-130b화RUNX3mRNA재위암조직중적표체,병평개miR-130b대억암기인RUNX3표체적영향.방법 용형광정량PCR기술검측40대위암조직급기대응적암방정상위점막조직중miR-130b화RUNX3mRNA적표체량；용면역조직화학SP법검측RUNX3단백적표체.결과 위암조직중miR-130b적표체현저고우대응적암방조직(2.18±3.75)비(2.59±3.45),P＜0.05;이위암조직중RUNX3mRNA적표체현저저우대응적암방조직(8.76±2.82)비(7.58±2.87),P ＜0.05.miR-130b화RUNX3mRNA적표체여위암적림파결전이、림상분기유관(P＜0.05),이여환자적년령、성별、종류대소、원처전이、유무침윤주변기관조직무관(균P ＞0.05).miR-130b적표체여RUNX3단백적핵표체、세포핵+세포질적표체존재부상관(P＜0.05),이여RUNX3mRNA、RUNX3단백적세포질표체무상관성(P＞0.05).결론 miR-130b화RUNX3mRNA적이상표체여위암예후유관；miR-130b가능통과감소RUNX3단백적표체수평도치종류적발생.
Objective To explore the expression of miR-130b and RUNX3mRNA in human gastric carcinoma and the clinical significance. Methods The expression of miR-130b and RUNX3mRNA were detected by RT-PCR in 40 cases of gastric carcinoma and corresponding normal mucosa tissue. The expression of RUNX3 protein was determined by immunohistochemistry SP method. Results The expression of miR-130b was significantly up-regulated in gastric carcinoma than that in the adjacent normal gastric mucosa tissues (2.18 ± 3.75 ) vs.( 2.59 ± 3.45 ),P ＜ 0.05 ; The expression of RUNX3mRNA in gastric carcinoma tissues was significantly lower than that in adjacent normal gastric mucosa tissues( 8.76 ±2.82) vs.( 7.58 ± 2.87 ),P ＜ 0.05.The expression of miR-130b and RUNX3mRNA were positively correlated with lymph node metastasis and clinical stage ( P ＜ 0.05 ) ; No significant association was found between the expression and age,gender,tumor size,distant metastasis and depth of tumor invasion ( P ＞0.05 ).The expression of miR-130b was negatively correlated with RUNX3 protein expression in nuclei and cytoplasm (P ＜ 0.05 ). Conclusions Abnormal expression of miR-130b and RUNX3mRNA correlates with prognosis of gastric carcinoma; Decreased RUNX3 protein expression may contribute to tumourigenesis.
