中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2011年
11期
841-845
,共5页
肺纤维化%受体,补体%多态性,限制性片段长度
肺纖維化%受體,補體%多態性,限製性片段長度
폐섬유화%수체,보체%다태성,한제성편단장도
Pulmonary fibrosis%Receptors,complement%Polymorphism,restriction fragment length
目的 探讨红细胞补体受体1(CR1)密度基因多态性及CR1基因A3650G位点多态性与特发性肺纤维化(IPF)易感性的关系,CR1的HindⅢ密度相关基因组多态性与红细胞膜上CR1数量表达水平的关系.方法 选择2009年11月至2010年12月在河北医科大学第二医院确诊为特发性肺纤维化汉族患者64例(IPF组)及54名同期河北医科大学第二医院健康汉族体检者作为健康对照组.应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)及聚合酶链反应(PCR)检测两组CR1的HindⅢ密度相关基因组多态性以及CR1基因A3650G多态位点的基因型；采用流式细胞术检测CR1在红细胞膜上的数量表达水平.结果 CR1HindⅢ密度相关基因基因型HH型、HL型和LL型在IPF组的分布频率分别为32.8％(21/64)、46.9％(30/64)和20.3％(13/64),在健康对照组分布频率分别为72.2％(39/54)、25.9％(14/54)和1.9％(1/54),差异有统计学意义(x2=15.516,P＜0.05).IPF组与健康对照组HL+ LL基因型优势比OR值为5.32(x2=18.20,P＜0.05).CR1基因A3650G位点的等位基因频率在IPF患者和健康对照组之间比较,总体分布差异无统计学意义(x2=1.094,P＞0.05).CR1在IPF组红细胞上的平均荧光强度为13.46±3.86,与健康对照组(24.33±3.84)比较,差异有统计学意义(t=15.288,P＜0.05).两组CR1基因HindⅢ-RFLP高表达型的红细胞CR1数量表达水平高于中表达型(t=9.973,P＜0.05),中表达型高于低表达型(t=4.359,P＜0.05).IPF组CR1基因HindⅢ-RFLP高表达型、中表达型、低表达型的红细胞CR1数量表达水平均低于健康对照人群(均P＜0.05).结论 红细胞CR1数量表达水平既受CR1基因HindⅢ密度相关基因组多态性遗传控制又受后天因素的影响.CR1基因HL型和LL型人群可能是特发性肺纤维化的易感人群,携带L等位基因的个体存在对特发性肺纤维化的易感性.
目的 探討紅細胞補體受體1(CR1)密度基因多態性及CR1基因A3650G位點多態性與特髮性肺纖維化(IPF)易感性的關繫,CR1的HindⅢ密度相關基因組多態性與紅細胞膜上CR1數量錶達水平的關繫.方法 選擇2009年11月至2010年12月在河北醫科大學第二醫院確診為特髮性肺纖維化漢族患者64例(IPF組)及54名同期河北醫科大學第二醫院健康漢族體檢者作為健康對照組.應用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)及聚閤酶鏈反應(PCR)檢測兩組CR1的HindⅢ密度相關基因組多態性以及CR1基因A3650G多態位點的基因型；採用流式細胞術檢測CR1在紅細胞膜上的數量錶達水平.結果 CR1HindⅢ密度相關基因基因型HH型、HL型和LL型在IPF組的分佈頻率分彆為32.8％(21/64)、46.9％(30/64)和20.3％(13/64),在健康對照組分佈頻率分彆為72.2％(39/54)、25.9％(14/54)和1.9％(1/54),差異有統計學意義(x2=15.516,P＜0.05).IPF組與健康對照組HL+ LL基因型優勢比OR值為5.32(x2=18.20,P＜0.05).CR1基因A3650G位點的等位基因頻率在IPF患者和健康對照組之間比較,總體分佈差異無統計學意義(x2=1.094,P＞0.05).CR1在IPF組紅細胞上的平均熒光彊度為13.46±3.86,與健康對照組(24.33±3.84)比較,差異有統計學意義(t=15.288,P＜0.05).兩組CR1基因HindⅢ-RFLP高錶達型的紅細胞CR1數量錶達水平高于中錶達型(t=9.973,P＜0.05),中錶達型高于低錶達型(t=4.359,P＜0.05).IPF組CR1基因HindⅢ-RFLP高錶達型、中錶達型、低錶達型的紅細胞CR1數量錶達水平均低于健康對照人群(均P＜0.05).結論 紅細胞CR1數量錶達水平既受CR1基因HindⅢ密度相關基因組多態性遺傳控製又受後天因素的影響.CR1基因HL型和LL型人群可能是特髮性肺纖維化的易感人群,攜帶L等位基因的箇體存在對特髮性肺纖維化的易感性.
목적 탐토홍세포보체수체1(CR1)밀도기인다태성급CR1기인A3650G위점다태성여특발성폐섬유화(IPF)역감성적관계,CR1적HindⅢ밀도상관기인조다태성여홍세포막상CR1수량표체수평적관계.방법 선택2009년11월지2010년12월재하북의과대학제이의원학진위특발성폐섬유화한족환자64례(IPF조)급54명동기하북의과대학제이의원건강한족체검자작위건강대조조.응용취합매련반응-한제성편단장도다태성(PCR-RFLP)급취합매련반응(PCR)검측량조CR1적HindⅢ밀도상관기인조다태성이급CR1기인A3650G다태위점적기인형；채용류식세포술검측CR1재홍세포막상적수량표체수평.결과 CR1HindⅢ밀도상관기인기인형HH형、HL형화LL형재IPF조적분포빈솔분별위32.8％(21/64)、46.9％(30/64)화20.3％(13/64),재건강대조조분포빈솔분별위72.2％(39/54)、25.9％(14/54)화1.9％(1/54),차이유통계학의의(x2=15.516,P＜0.05).IPF조여건강대조조HL+ LL기인형우세비OR치위5.32(x2=18.20,P＜0.05).CR1기인A3650G위점적등위기인빈솔재IPF환자화건강대조조지간비교,총체분포차이무통계학의의(x2=1.094,P＞0.05).CR1재IPF조홍세포상적평균형광강도위13.46±3.86,여건강대조조(24.33±3.84)비교,차이유통계학의의(t=15.288,P＜0.05).량조CR1기인HindⅢ-RFLP고표체형적홍세포CR1수량표체수평고우중표체형(t=9.973,P＜0.05),중표체형고우저표체형(t=4.359,P＜0.05).IPF조CR1기인HindⅢ-RFLP고표체형、중표체형、저표체형적홍세포CR1수량표체수평균저우건강대조인군(균P＜0.05).결론 홍세포CR1수량표체수평기수CR1기인HindⅢ밀도상관기인조다태성유전공제우수후천인소적영향.CR1기인HL형화LL형인군가능시특발성폐섬유화적역감인군,휴대L등위기인적개체존재대특발성폐섬유화적역감성.
Objective To explore the association between the erythrocyte CR1 genomic density polymorphism,A3650G site polymorphism and the susceptibility of idiopathic pulmonary fibrosis (IPF) ; and to investigate the correlation between the Hind Ⅲ density polymorphism of CR1 gene and the quantitative levels of E-CR1 in IPF patients.Methods Blood samples from IPF patients (n =64 ) and ethnically matched healthy controls ( n =54 ) were taken from a population-based case-control association study.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to identify the genotype of the HindⅢ restriction fragment length polymorphism of CR1 gene and SNP A3650G in two groups.Quantitative expression of CR1 on RBC membrane surface was detected by flow cytometry.Results The genotype frequencies of HH,HL and LL were 32.8 ％ (21/64),46.9 ％ (30/64) and 20.3 ％(13/64) respectively in the IPF group,and 72.2 ％ (39/54),25.9 ％ (14/54) and 1.9 ％ (1/54)respectively in the controls.The distribution of genotype between the two groups was significantly different (x2 =15.516,P ＜0.05).HL + LL genotype for the HindⅢ polymorphism was more common in patients with IPF compared to the controls with an OR =5.32 ( x2 =18.20,P ＜ 0.05 ). Compared the allele frequency of A3650G sites in the IPF group with that in the control group,there was no difference from distribution in the two groups ( x2 =1.094,P ＞ 0.05 ).The mean CR1/E numbers observed in the IPF patients was 13.46 + 3.86,and the mean CR1/E in normal individuals was 24.33 ± 3.84 ( t =15.288,P ＜0.05 vs IPF group).The levels of E-CR1 in both IPF patients and healthy controls HH genotype for E-CR1 Hind Ⅲ-RFLP were significantly higher than HL genotype for E-CR1 HindⅢ-RFLP( t =9.973,P ＜ 0.05 ),and the levels of E-CR1 in both groups HL genotype for E-CR1 HindⅢ-RFLP were significantly higher than LL genotype for E-CR1 HindⅢ-RFLP( t =9.973,P ＜ 0.05 ).The levels of HH,HL and LL genotypes for E-CR1 HindⅢ-RFLP in the IPF group were significantly lower than those in the control group,respectively (P ＜ 0.05,on average).Conclusion The quantitative levels of CR1 on erythrocyte membrane was not only determined by the genetic background of E-CR1 HindⅢ-RFLP but also by the acquired predisposition.HL and LL genotypes of CR1 gene may be associated with IPF,and as a result individuals carrying the L allele might be a susceptible population for IPF.
