CAJ | 학술논문

目的探讨富氧吸人对大鼠模拟急性高原肺水肿的防护作用.方法将44只健康雄性Wistar大鼠随机分为4组,分别为对照组、低压低氧组、连续富氧组、夜间富氧组,每组11只.除对照组外,其余3组均置于模拟6000 m海拔高度的低压舱中48 h.富氧干预48 h后,测定各组大鼠的肺湿干比、肺泡灌洗液(bronchoalveolar lavage fluid,BALF)血清和肺组织匀浆一氧化氮(nitric oxide,NO)含量、一氧化氮合酶(nitric oxide synthase,NOS)及肺组织病理学改变.结果与对照组相比,低压低氧组大鼠BALF蛋白浓度及肺湿干比显著升高(P＜0.01)、血清及肺组织匀浆中NO 含量、NOS降低(P＜0.01).病理学显示低压低氧组大鼠有肺泡间隔增宽、间质充血、炎细胞浸润等间质性肺水肿表现.连续富氧及夜间富氧均可降低肺泡灌洗液的蛋白浓度及肺湿干比,提高大鼠血清和肺组织内的NO含量、NOS活性,减轻肺泡间隔增厚、炎细胞浸润及肺泡出血,且连续富氧组与夜间富氧组各项指标差异无统计学意义.结论富氧吸人可以通过提高体内的NO含量、NOS活性来防护大鼠急进高原肺水肿,夜间富氧与连续富氧防护效果无明显差别.
목적 탐토부양흡인대대서모의급성고원폐수종적방호작용.방법 장44지건강웅성Wistar대서수궤분위4조,분별위대조조、저압저양조、련속부양조、야간부양조,매조11지.제대조조외,기여3조균치우모의6000 m해발고도적저압창중48 h.부양간예48 h후,측정각조대서적폐습간비、폐포관세액(bronchoalveolar lavage fluid,BALF)혈청화폐조직균장일양화담(nitric oxide,NO)함량、일양화담합매(nitric oxide synthase,NOS)급폐조직병이학개변.결과 여대조조상비,저압저양조대서BALF단백농도급폐습간비현저승고(P＜0.01)、혈청급폐조직균장중NO 함량、NOS강저(P＜0.01).병이학현시저압저양조대서유폐포간격증관、간질충혈、염세포침윤등간질성폐수종표현.련속부양급야간부양균가강저폐포관세액적단백농도급폐습간비,제고대서혈청화폐조직내적NO함량、NOS활성,감경폐포간격증후、염세포침윤급폐포출혈,차련속부양조여야간부양조각항지표차이무통계학의의.결론 부양흡인가이통과제고체내적NO함량、NOS활성래방호대서급진고원폐수종,야간부양여련속부양방호효과무명현차별.
Objective To investigate the protective effects of oxygen enrichment inhalation on preventing acute high altitude pulmonary edema (HAPE) of rats and compare the effects of various oxygen inhalations. Methods Forty-four male Wistar rats were randomly and averagely divided into control, hypoxia, fulltime oxygen enrichment and nocturnal oxygen enrichment groups. Rats with various inhalations were exposed to simulated 6000 m in hypobaric chamber for 48 h except control group..The wet to dry ratio (W/D), protein concentration of bronchoalveolar lavage fluid (BALF),nitric oxide (NO) content and nitric oxide synthase (NOS) activity in serum and lung homogenate were measured, and lung histological change was determined. Results Comparing with control group, hypoxia group showed significantly increased W/D, BALF protein concentration and decreased NO level and NOS activity (P＜0.01). Interstitial pulmonary edema, haemorrhage and neutrophil infiltration were found in hypoxia group. W/D and BALF protein concentration both in fulltime oxygen enrichment and nocturnal oxygen enrichment groups were decreased compared with those in hypoxia group (P＜0. 05), while the level of NO and the activity of NOS were increased (P＜0. 01).Conclusions Oxygen enrichment inhalation has the protective effects on preventing HAPE by improving NO level and NOS activity. There is no significant different protective effect between fulltime oxygen enrichment and nocturnal oxygen enrichment groups.