CAJ | 학술논문

目的研究康复训练对大鼠脑梗死灶周同白质Nogo-A表达的影响.方法采用完全随机化方法将60只Wistar大鼠分成康复训练组和造模对照组,每组30只,应用Longa颈外动脉线栓法制备大鼠大脑中动脉闭塞模型.康复训练组大鼠每天进行滚筒、平衡木、转棒及网屏训练,时间共1 h;造模对照组置于普通笼中自由活动.每组分别于造模后3,7,14,21和28 d 5个时间点随机选取6只大鼠进行行为学评估后处死,取脑组织采用免疫组织化学方法观察每个时间点脑梗死灶周围白质Nogo-A的表达.结果 ①康复训练组术后14,21和28 d行为学评分明显低于造模对照组,差异具有统计学意义(P<0.05).②2组大鼠Nogo-A阳性细胞于脑缺血7 d后开始增加,21 d达高峰;脑缺血14 d,21 d和28 d时间点,康复训练组Nogo-A阳性细胞的表达水平明显低于造模对照组,差异具有统计学意义(P<0.05).结论康复训练能通过减少脑梗死大鼠梗死灶周围Nogo-A的表达,减低其对神经轴突生长抑制作用,从而促进脑梗死后神经功能的恢复.
목적 연구강복훈련대대서뇌경사조주동백질Nogo-A표체적영향.방법 채용완전수궤화방법장60지Wistar대서분성강복훈련조화조모대조조,매조30지,응용Longa경외동맥선전법제비대서대뇌중동맥폐새모형.강복훈련조대서매천진행곤통、평형목、전봉급망병훈련,시간공1 h;조모대조조치우보통롱중자유활동.매조분별우조모후3,7,14,21화28 d 5개시간점수궤선취6지대서진행행위학평고후처사,취뇌조직채용면역조직화학방법관찰매개시간점뇌경사조주위백질Nogo-A적표체.결과 ①강복훈련조술후14,21화28 d행위학평분명현저우조모대조조,차이구유통계학의의(P<0.05).②2조대서Nogo-A양성세포우뇌결혈7 d후개시증가,21 d체고봉;뇌결혈14 d,21 d화28 d시간점,강복훈련조Nogo-A양성세포적표체수평명현저우조모대조조,차이구유통계학의의(P<0.05).결론 강복훈련능통과감소뇌경사대서경사조주위Nogo-A적표체,감저기대신경축돌생장억제작용,종이촉진뇌경사후신경공능적회복.
Objective To study the effects of rehabilitation training on the expression of Nogo-A around the area of cerebral infraction using rats. Methods A total of 60 Wistar rats were randomly divided into a reha- bilitation group and a control group after an experimental cerebral infarction had been established in them. The ani- mals in the rehabilitation group were given exercise with a rotating bar, a balance beam and a rolling cage for one hour daily, while those in the control group were caged without any abnormal exercise. Nogo-A expression in the ar- ea surrounding the infarcts was detected by imunohistochemical techniques at the 3rd, 7th, 14th, 21st and 28th day after infarction. Meanwhile, neurobehavioral evaluations were also conducted. Results The animals in the rehabilitation group scored much lower than the controls in the behavioral evaluations at the 14th, 21st and 28th day. The expression of Nogo-A in tissues around the infracted area increased by the 7th day and peaked at the 21st day in both groups, but the expression of Nogo-A was significantly stronger in the rehabilitation group at the 14th, 21st and 28th days. Conclusion Rehabilitation training decreased the expression of Nogo-A in the brain of rats after infarction. This may have important implications for the functional recovery of the central nervous system.