CAJ | 학술논문

目的分析1株柯萨奇病毒B3型(CoxB3)新分离株FY-19的基因特性和生物性状,为进一步探讨其引起手足口病(HFMD)重症表现的机理提供线索.方法采用Lim Benyesh-Melnick组合血清方案对1株分离自2008年中国阜阳地区重症HFMD男性患儿的FY-19病毒株进行血清学鉴定,以全基因序列测定分析该病毒株的遗传特征,结合病毒增殖动力学和噬斑形成实验分析FY-19病毒的生物学特性,通过乳鼠脑内病毒接种研究该毒株的致病性特征.结果血清学鉴别确定FY-19毒株为CoxB3病毒;与标准株相比,FY-19株在3'、5'非编码区和编码区的差异分别为达到23.0%、16.5%和32.1%,与我国分离于非HFMD患者的毒株相比也存在较大差异(非编码区和编码区的差异分别为13.5%和25.0%);FY-19株能在14 h内达到增殖高峰,且其在小鼠体内引起心肌病变的致病性显示出明显的致死能力.结论 FY-19株的生物学特性与标准毒株之间存在较大的差异,对该变异株的进一步分析将为了解其在HFMD的特殊的致病机理提供帮助.
목적 분석1주가살기병독B3형(CoxB3)신분리주FY-19적기인특성화생물성상,위진일보탐토기인기수족구병(HFMD)중증표현적궤리제공선색.방법 채용Lim Benyesh-Melnick조합혈청방안대1주분리자2008년중국부양지구중증HFMD남성환인적FY-19병독주진행혈청학감정,이전기인서렬측정분석해병독주적유전특정,결합병독증식동역학화서반형성실험분석FY-19병독적생물학특성,통과유서뇌내병독접충연구해독주적치병성특정.결과 혈청학감별학정FY-19독주위CoxB3병독;여표준주상비,FY-19주재3'、5'비편마구화편마구적차이분별위체도23.0%、16.5%화32.1%,여아국분리우비HFMD환자적독주상비야존재교대차이(비편마구화편마구적차이분별위13.5%화25.0%);FY-19주능재14 h내체도증식고봉,차기재소서체내인기심기병변적치병성현시출명현적치사능력.결론 FY-19주적생물학특성여표준독주지간존재교대적차이,대해변이주적진일보분석장위료해기재HFMD적특수적치병궤리제공방조.
Objective To analyze the genetic and biological characters of a new isolate of coxsackievirus B3( CoxB3), i. e. FY-19 strain, and investigate its mechanistic role in causing different clinical symptoms of hand-foot-mouth disease (HFMD). Methods FY-19 strain, isolated from a patient with severe clinical symptoms from Fuyang, China in 2008, was identified by the serological parameters via the Lim Benyesh-Melnick (LBM) antiserum pools. Its genotype was further characterized by sequencing the whole genome. And its biological characters were also examined by proliferation kinetic and pathogenetic analysis. Results FY-19 strain was identified as CoxB3 showing 23.0%, 16. 5% and 32. 1% difference with Nancy strain in 3'-, 5'-nonconding and coding regions respectively. FY-19 also showed a high homology with other HFMD-related CoxB3 isolates in China. But its homology with non-HFMD-related CoxB3 isolates was lower (13. 5% and 25.0% difference in 3'-NCR and coding region respectively). The viral replication kinetic analysis suggested that the FY-19 proliferation increased rapidly and peaked at 14 hours postinfection. In pathological analysis, FY-19 strain induced mortal pathology in sucking mice. Conclusion Differences in genetic and biological characters exist between FY-19 and Nancy strains. Further analysis on the pathogenesis of this variant may aid in elucidating the mechanisms of HFMD.