CAJ | 학술논문

目的观察不同治疗阶段大肠癌患者T淋巴细胞亚群CD+4细胞中,Th1/Th2类因子水平的变化,为大肠癌的生物治疗提供依据.方法分离外周血淋巴细胞,经刺激增加细胞内因子表达,荧光标记特异性抗细胞因子单克隆抗体,特异性抗原抗体结合,用流式细胞术分析、测定特异性细胞因子表达水平.结果大肠癌患者在不同的治疗阶段,Th1类因子的变化较明显,差异均有统计学意义;Th2类因子变化无显著性.IFN-γ/IL-4的比值变化只在生物治疗前后差异有统计学意义.结论大肠癌患者在不同治疗阶段,Th1类因子水平的变化对生物治疗具有明显的指导意义;Th2类因子水平无指导意义.其中IFN-γ/IL-4比值的变化在生物治疗中具有较好的指导性,合理的生物治疗可逆转Th1/Th2因子的抗肿瘤抑制作用.
목적 관찰불동치료계단대장암환자T림파세포아군CD+4세포중,Th1/Th2류인자수평적변화,위대장암적생물치료제공의거.방법 분리외주혈림파세포,경자격증가세포내인자표체,형광표기특이성항세포인자단극륭항체,특이성항원항체결합,용류식세포술분석、측정특이성세포인자표체수평.결과 대장암환자재불동적치료계단,Th1류인자적변화교명현,차이균유통계학의의;Th2류인자변화무현저성.IFN-γ/IL-4적비치변화지재생물치료전후차이유통계학의의.결론 대장암환자재불동치료계단,Th1류인자수평적변화대생물치료구유명현적지도의의;Th2류인자수평무지도의의.기중IFN-γ/IL-4비치적변화재생물치료중구유교호적지도성,합리적생물치료가역전Th1/Th2인자적항종류억제작용.
Objective To observe the levels of Th1 and Th2 type cytokines of peripheral blood CD+4 T lymphocytes at different stage of treatment of colonic cancer,in order to find out the laboratory evidence of tumor immunotherapy.Methods The peripheral blood lymphocytes were stimulated by stimulator in order to enhance the expression of cytokines.Then specific fluorescent-conjugated monoclonal antibodies were added to combine with its specific antigens.Cytokines were detected by flow cytometry.Results At different stage of treatment of colonic cancer,the levels of Th1 type cytokines of each stage was significant.The levels of Th2 type cytokines at each stage were not significant.The IFN-γ//IL-4 ratio were significantly different during tumor immunothempy.Conclusion At different stage of treatment of colonic cancer,the levels of Th,type cytokines were obviously associated with treatment.The levels of Th2 type cytokines had no association with treatment.Among them,the changes of Th1/Th2 ratio (IFN-γ/IL-4) had more correlation with tumor immunotherapy.So the reasonable immunotherapy can reverse Th1/Th2 ratio and immune inhibition.