青岛大学医学院学报
青島大學醫學院學報
청도대학의학원학보
ACTA ACADEMIAE MEDICINAE QINGDAO UNIVERSITATIS
2006年
1期
27-31
,共5页
王岭%高晓玉%张美增%孙成云%郭云良
王嶺%高曉玉%張美增%孫成雲%郭雲良
왕령%고효옥%장미증%손성운%곽운량
脑缺血%基因表达%巢蛋白%干细胞因子%神经细胞黏附分子类%大鼠,Sprague-Dawley
腦缺血%基因錶達%巢蛋白%榦細胞因子%神經細胞黏附分子類%大鼠,Sprague-Dawley
뇌결혈%기인표체%소단백%간세포인자%신경세포점부분자류%대서,Sprague-Dawley
brain ischemia%gene expression%Nestin%stem cell factor%neural cell adhesion molecules%rats,Sprague-Dawley
目的观察大鼠脑缺血再灌注后神经细胞巢蛋白(Nestin)、干细胞因子(SCF)和神经细胞黏附分子(NCAM)基因的表达.方法成年健康雌性SD大鼠36只,以线栓法建立大脑中动脉缺血再灌注模型,随机分为缺血1.5 h再灌注2 h、6 h、12 h、24 h、2 d、3 d、7 d、14 d组和假手术组,每组4只.应用原位杂交技术检测脑缺血再灌注后脑组织Nestin、SCF和NCAM mRNA的表达.结果假手术组皮质和纹状体区Nestin、SCF和NCAMmRNA均有微弱表达.脑缺血再灌注后Nestin mRNA表达增高,皮质除再灌注2 h以外、纹状体除再灌注2、6 h以外,其余各时间点均明显高于假手术组.SCF mRNA表达,皮质除再灌注2、6、12 h以外,纹状体除再灌注2、6 h以外,其余各时间点均明显高于假手术组.NCAM mRNA于再灌注2 h后在皮质和纹状体区开始表达,分别于再灌注12 h和1 d达高峰,7 d后逐渐减少,14 d降至假手术组水平.结论脑缺血再灌注后SCF mRNA表达可能具有促进神经干细胞增殖作用,NCAM表达可能参与了损伤后脑组织的修复过程.
目的觀察大鼠腦缺血再灌註後神經細胞巢蛋白(Nestin)、榦細胞因子(SCF)和神經細胞黏附分子(NCAM)基因的錶達.方法成年健康雌性SD大鼠36隻,以線栓法建立大腦中動脈缺血再灌註模型,隨機分為缺血1.5 h再灌註2 h、6 h、12 h、24 h、2 d、3 d、7 d、14 d組和假手術組,每組4隻.應用原位雜交技術檢測腦缺血再灌註後腦組織Nestin、SCF和NCAM mRNA的錶達.結果假手術組皮質和紋狀體區Nestin、SCF和NCAMmRNA均有微弱錶達.腦缺血再灌註後Nestin mRNA錶達增高,皮質除再灌註2 h以外、紋狀體除再灌註2、6 h以外,其餘各時間點均明顯高于假手術組.SCF mRNA錶達,皮質除再灌註2、6、12 h以外,紋狀體除再灌註2、6 h以外,其餘各時間點均明顯高于假手術組.NCAM mRNA于再灌註2 h後在皮質和紋狀體區開始錶達,分彆于再灌註12 h和1 d達高峰,7 d後逐漸減少,14 d降至假手術組水平.結論腦缺血再灌註後SCF mRNA錶達可能具有促進神經榦細胞增殖作用,NCAM錶達可能參與瞭損傷後腦組織的脩複過程.
목적관찰대서뇌결혈재관주후신경세포소단백(Nestin)、간세포인자(SCF)화신경세포점부분자(NCAM)기인적표체.방법성년건강자성SD대서36지,이선전법건립대뇌중동맥결혈재관주모형,수궤분위결혈1.5 h재관주2 h、6 h、12 h、24 h、2 d、3 d、7 d、14 d조화가수술조,매조4지.응용원위잡교기술검측뇌결혈재관주후뇌조직Nestin、SCF화NCAM mRNA적표체.결과가수술조피질화문상체구Nestin、SCF화NCAMmRNA균유미약표체.뇌결혈재관주후Nestin mRNA표체증고,피질제재관주2 h이외、문상체제재관주2、6 h이외,기여각시간점균명현고우가수술조.SCF mRNA표체,피질제재관주2、6、12 h이외,문상체제재관주2、6 h이외,기여각시간점균명현고우가수술조.NCAM mRNA우재관주2 h후재피질화문상체구개시표체,분별우재관주12 h화1 d체고봉,7 d후축점감소,14 d강지가수술조수평.결론뇌결혈재관주후SCF mRNA표체가능구유촉진신경간세포증식작용,NCAM표체가능삼여료손상후뇌조직적수복과정.
Objective To study the gene expressions of Nestin, stem cell factor (SCF) and neural cell adhesion molecule (NCAM) in neurons after ischemia-reperfusion injury in rat brain. Methods Thirty-six adult female rats were induced by intraluminal middle cerebral artery (MCA) occlusion, and divided into ischemia-1.5 h group and reperfusion 2 h-, 6 h-, 12 h-, 1 d-, 2 d-, 3 d-, 7 d-, 14 d- groups and sham-operated group (n=4), with a nylon monofilament suture. In situ hybridization was used to examine the expression of Nestin, SCF and NCAM mRNA. Results There were basic expression of Nestin, SCF and NCAM mRNA in brain tissue of sham-operated group. Nestin mRNA expression in cortex and striatum increased markedly in ischemic hemisphere compared with sham-operated group except reperfusion 2 h in cortex and 2 h, 6 h in striatum. SCF mRNA expression in cortex and striatum increased significantly in ischemic hemisphere compared with sham-operated group except reperfusion 2 h, 6 h, 12 h in cortex and 2 h, 6 h in striatum. NCAM mRNA expression was observed after reperfusion 2 h,peaked at 12 h and 1 d in cortex and striatum, respectively, and decreased to the basic level of sham-operated group at 14 d. Conclusion The results indicate that SCF expression might enhance neural stem cell proliferation following ischemia reperfusion. The increasing NCAM expression might play an important role in the neural repair of injured brain tissues.