CAJ | 학술논문

42只双侧前胸壁荷瘤的VX2兔模型(共计84个肿瘤)随机分为治疗组(n=32)和对照组(n=10).治疗组静脉给予4mg/kg顺铂前(Pre-therapy)和给药后95-100min(Day 0)、Day 1、Day7、Day 14行PET/CT显像;对照组不给化疗药物,其余与实验组相同.取葡萄糖摄取最大值(SUVmax)进行分析,CT测量肿瘤大小.按肿瘤体积分组,Day 7时治疗组肿瘤体积增长>1倍则为不敏感,反之为敏感.结果显示:(1)Day 0敏感组SUVmax减低率为(-48.96±12.27)%,而不敏感组和对照组为(21.26±18.26)%和(7.16±13.47)%,三组SUVmax变化有显著差异(P<0.05).(2)Pre-therapy、Day 0、Day 1、Day 14两组肿瘤体积无显著性差异(P>0.05),但Day 7敏感组肿瘤体积小于不敏感组及对照组,差异有显著性(P<0.05).(3)Day 7、Day 14敏感组肿瘤坏死率大于不敏感组及对照组,差异有显著性(P<0.05).(4)HE染色观察不同时间点切除的肿瘤标本发现:Day 7、Day 14时敏感组肿瘤细胞数少而炎性和坏死细胞数增加.表明根据化疗药物给予后FDG减低程度,18F-FDG PET/CT能在体、早期、灵敏检出肿瘤对化疗药物的敏感性.
42지쌍측전흉벽하류적VX2토모형(공계84개종류)수궤분위치료조(n=32)화대조조(n=10).치료조정맥급여4mg/kg순박전(Pre-therapy)화급약후95-100min(Day 0)、Day 1、Day7、Day 14행PET/CT현상;대조조불급화료약물,기여여실험조상동.취포도당섭취최대치(SUVmax)진행분석,CT측량종류대소.안종류체적분조,Day 7시치료조종류체적증장>1배칙위불민감,반지위민감.결과현시:(1)Day 0민감조SUVmax감저솔위(-48.96±12.27)%,이불민감조화대조조위(21.26±18.26)%화(7.16±13.47)%,삼조SUVmax변화유현저차이(P<0.05).(2)Pre-therapy、Day 0、Day 1、Day 14량조종류체적무현저성차이(P>0.05),단Day 7민감조종류체적소우불민감조급대조조,차이유현저성(P<0.05).(3)Day 7、Day 14민감조종류배사솔대우불민감조급대조조,차이유현저성(P<0.05).(4)HE염색관찰불동시간점절제적종류표본발현:Day 7、Day 14시민감조종류세포수소이염성화배사세포수증가.표명근거화료약물급여후FDG감저정도,18F-FDG PET/CT능재체、조기、령민검출종류대화료약물적민감성.
This study is to explore the feasibility of using 18F-FDG PET/CT as an in vivo individual chemosensitivitytesting method.Forty-two VX2 rabbits bearing a total of 84 tumors were randomized into treatment group (n=32) and control group (n=10).18F-FDG PET/CT was performed the day before intravenous administration of cisplatin (4 mg/kg) and at 95-100 min (Day 0),Day 1,Day 7,and Day 14 afterward.The control group,without cisplatin ad-ministration,received the 18F-FDG PET/CT imaging at the same time points.Maximum standardized uptake value (SUV) was analyzed.The animals on Day 7 with a tumor volume of at least twice as larger than Day 0,were regarded as the sensitive group (SG),while the others the insensitive group (ISG).The results showed that a significant differ-ence (P<0.05) in SUV on Day 0 between SG and ISG,with SUV decrease rate of (-48.96±12.27)%,(21.26±18.26)%and (7.16±13.47)% for SG,ISG and the control,respectively.No significant difference in tumor volume was found among the three groups pre-therapy and on Day 0 and Day 1.On Day 7,however,the tumor volume with SG was smaller than with ISG,in a significant difference of P<0.05,while no significant difference was seen between ISG and the control on Day 7.On Day 14,no significant difference was observed among the tumor volume of the three groups (P>0.05).On Day 7 and Day 14,significant differences were seen in tumor necrosis rate in SG and ISG (P<0.05),but no significant differences were seen between ISG and the control.Paraffin section stained with haema-toxylin and eosin of sections obtained at different time showed that the number of viable tumor cells in sensitive group diminished than ISG and the control,and inflammation and necrotic cells were increased on Day 7 and Day 14.The study showed that 18F-FDG PET/CT can be used as an in vivo chemosensitivity testing method.According to the decrease rate when cisplatin adminstration,PET can early in vivo sensitively differentiate the sensitive and insensitive tumors.