CAJ | 학술논문

目的采用已建立的未成熟睾丸组织异体异位移植模型,研究受体小鼠激素水平变化对未成熟睾丸组织移植物生长发育的影响.方法以免疫缺陷小鼠为受体,将新生1～2d龄昆明小鼠睾丸移植到受体小鼠背部皮下.在移植后不同阶段取出移植物,进行组织形态学观察,分析移植物中生精细胞的发育情况,同时采用放射免疫分析法检测受体动物血清中睾酮(T)、促卵泡刺激素(FSH)和促黄体生成素(LH)水平,并以同龄正常昆明小鼠作对照.结果受体小鼠血清中LH水平没有明显波动,而T和FSH浓度在移植的前8周呈逐渐上升趋势,从9周以后开始下降,且显著低于正常受体内睾酮和FSH浓度,与组织学中所观察到的生精上皮受损程度的增加是同步的.结论以上结果提示,应注意获取移植物中精子的最佳时间.对于小鼠来说,从新生小鼠睾丸移植物中获取精子的最佳时间是术后5-8周.
목적 채용이건립적미성숙고환조직이체이위이식모형,연구수체소서격소수평변화대미성숙고환조직이식물생장발육적영향.방법 이면역결함소서위수체,장신생1～2d령곤명소서고환이식도수체소서배부피하.재이식후불동계단취출이식물,진행조직형태학관찰,분석이식물중생정세포적발육정황,동시채용방사면역분석법검측수체동물혈청중고동(T)、촉란포자격소(FSH)화촉황체생성소(LH)수평,병이동령정상곤명소서작대조.결과 수체소서혈청중LH수평몰유명현파동,이T화FSH농도재이식적전8주정축점상승추세,종9주이후개시하강,차현저저우정상수체내고동화FSH농도,여조직학중소관찰도적생정상피수손정도적증가시동보적.결론 이상결과제시,응주의획취이식물중정자적최가시간.대우소서래설,종신생소서고환이식물중획취정자적최가시간시술후5-8주.
Objective To investigate the effect of hormone level of testosterone(T), FSH and LH in recipient mice on the development of spermatogenic cells in ectopic testicular grafts. Methods Neonatal Kun-ming mouse testes were inoculated into subcutaneous tissue of castrated nude mice. Grafts were then taken out at different time intervals for histological and morphological analysis to monitor the changes of spermatogenic cells. Serum T, FSH and LH level in recipient mice were measured by radioimmunoassay. Results There were no significant changes in LH level in recipient mice at different time point.There was an increasing trend in serum T and FSH level in recipients during 8 weeks after the testicular grafting, thereafter a decreasing trend was found. These changes were positively associated with the damage grades of seminiferous epithelia. Conclusion All data suggest that the best time point for retrieval of sperm from ectopic grafting of neonatal mouse testicular tissues was from 5th week to 8th week after inoculation.