CAJ | 학술논문

目的:探讨CpG ODN对透明带(四)抗原表位ZP~(121-140)合成肽诱导的免疫应答和免疫避孕效应影响.方法:应用人工合成ZP2~(121-140)表位肽,与20μg CpG ODN或等量完全弗氏佐剂(CFA)混悬后左胫前肌免疫雌性BALB/c小鼠,初次免疫后2、4、6周各加强免疫一次,共免疫四次.在每次加强免疫前和末次免疫后两周断尾取血,分离血清,分析特异性lgG抗体及非特异性细胞因子IFN-γ、TNF-Ⅱ,IL-10水平;收集小鼠阴道冲洗液,离心取上清,分析特异性IgA抗体水平.免疫小鼠生育实验结束后,取卵巢组织行病理学分析.结果:CpG ODN组诱导的特异性IgG和IgA水平较CFA组有增高趋势,但无显著性差异(P＞O.05).CpG ODN组诱导的非特异性IFN-γ和TNF-水平明显高于CFA组(P＜0.05);而CpG ODN组诱导的IL-10水平显著低于CFA 组(P＜0.05).两种佐剂对小鼠的受孕率影响没有显著性差异,但CpG ODN组孕鼠每胎产仔数明显低于CFA组(P＜O.05).病理学分析显示实验小鼠卵巢组织无病理性变化.结论:CpG ODN对Zp~(121-140)合成肽诱导的免疫应答和免疫避孕效应略优于CFA,更适合用于避孕疫苗佐剂研究.
목적:탐토CpG ODN대투명대(사)항원표위ZP~(121-140)합성태유도적면역응답화면역피잉효응영향.방법:응용인공합성ZP2~(121-140)표위태,여20μg CpG ODN혹등량완전불씨좌제(CFA)혼현후좌경전기면역자성BALB/c소서,초차면역후2、4、6주각가강면역일차,공면역사차.재매차가강면역전화말차면역후량주단미취혈,분리혈청,분석특이성lgG항체급비특이성세포인자IFN-γ、TNF-Ⅱ,IL-10수평;수집소서음도충세액,리심취상청,분석특이성IgA항체수평.면역소서생육실험결속후,취란소조직행병이학분석.결과:CpG ODN조유도적특이성IgG화IgA수평교CFA조유증고추세,단무현저성차이(P＞O.05).CpG ODN조유도적비특이성IFN-γ화TNF-수평명현고우CFA조(P＜0.05);이CpG ODN조유도적IL-10수평현저저우CFA 조(P＜0.05).량충좌제대소서적수잉솔영향몰유현저성차이,단CpG ODN조잉서매태산자수명현저우CFA조(P＜O.05).병이학분석현시실험소서란소조직무병이성변화.결론:CpG ODN대Zp~(121-140)합성태유도적면역응답화면역피잉효응략우우CFA,경괄합용우피잉역묘좌제연구.
Objective:To investigate the effect of CpG ODN on the immune responses and immune-contraception induced by ZP~(121-140) synthetic peptide.Methods: BALB/e mice were given an injection into the left tibialis anterior muscle of ZP~(121-140) synthetic peptide with 20 μg CpG ODN or CFA,then the mice were given other injections at 2,4,6 weeks using the same formulation.The mice' s blood was collected before each vaccination and after the last vaccination every 2 weeks.The specific IgG and IgA in sere and non-specific cytokines IFN-γ,TNF-α,IL-10 in vaginal mucosa were measured by ELISA.The ovarial pathological changes were undertaken using hemattxylin and eosin-stained paraffin sections.Results:The specific IgG in sera and IgA in vaginal mucosa induced by ZP~(121-140) synthetic peptide combined with CpG ODN were no more than those of ZP~(121-140) synthetic peptide combined with CFA.There were significant increases in IFN-γ,and TNF-α when CpG ODN was mixed with ZP121-140 synthetic peptide and the increase of CpG ODN was more significant than that of CFA.Otherwise there was a significant decrease in IL-10 when CpG ODN was mixed with ZP~(121-140) synthetic peptide and the decrease of CpG ODN was more significant than thai of CFA.There was no significant difference in the rate of pregnancy between CpG ODN group and CFA group,but the average number of birth mice in CpG ODN group was less than that in CFA group.No pathological changes were found in the ovaries of experimental mice.Conclusion: The adjuvant effect of CpG ODN is more advantageous than that of CFA in contraception vaccine research.