食品与生物技术学报
食品與生物技術學報
식품여생물기술학보
JOURNAL OF FOOD SCIENCE AND BIOTECHNOLOGY
2010年
1期
150-154
,共5页
4-烯-雄甾-3,17-二酮%l,4-二烯-雄甾-3,17-二酮%植物甾醇%生物转化
4-烯-雄甾-3,17-二酮%l,4-二烯-雄甾-3,17-二酮%植物甾醇%生物轉化
4-희-웅치-3,17-이동%l,4-이희-웅치-3,17-이동%식물치순%생물전화
AD%ADD%phytosterol%biotransformation
采用紫外线照射和NTG对实验室保存的一株对植物甾醇有降解能力的Mycobacterium sp. SH5进行诱变处理,得到一株雄甾-4-烯-3,17-二酮(Androst-4-end- 3,17-dione, AD)和雄甾-1,4-二烯-3,17-二酮(Androst -1,4-end-3,17-dione, ADD)分解酶 (9α-羟化酶) 缺陷型菌株Mycobacterium sp. SH5-52.对Mycobacterium sp. SH5-52降解植物甾醇侧链得到AD (D) 的培养基组成、接种量、温度、pH值和溶氧等发酵条件进行了研究.在最佳的发酵条件下,投料质量浓度为0.6 g/dL时,AD (D) 的转化率由原来的37.2%提高到47.6%左右.
採用紫外線照射和NTG對實驗室保存的一株對植物甾醇有降解能力的Mycobacterium sp. SH5進行誘變處理,得到一株雄甾-4-烯-3,17-二酮(Androst-4-end- 3,17-dione, AD)和雄甾-1,4-二烯-3,17-二酮(Androst -1,4-end-3,17-dione, ADD)分解酶 (9α-羥化酶) 缺陷型菌株Mycobacterium sp. SH5-52.對Mycobacterium sp. SH5-52降解植物甾醇側鏈得到AD (D) 的培養基組成、接種量、溫度、pH值和溶氧等髮酵條件進行瞭研究.在最佳的髮酵條件下,投料質量濃度為0.6 g/dL時,AD (D) 的轉化率由原來的37.2%提高到47.6%左右.
채용자외선조사화NTG대실험실보존적일주대식물치순유강해능력적Mycobacterium sp. SH5진행유변처리,득도일주웅치-4-희-3,17-이동(Androst-4-end- 3,17-dione, AD)화웅치-1,4-이희-3,17-이동(Androst -1,4-end-3,17-dione, ADD)분해매 (9α-간화매) 결함형균주Mycobacterium sp. SH5-52.대Mycobacterium sp. SH5-52강해식물치순측련득도AD (D) 적배양기조성、접충량、온도、pH치화용양등발효조건진행료연구.재최가적발효조건하,투료질량농도위0.6 g/dL시,AD (D) 적전화솔유원래적37.2%제고도47.6%좌우.
Mycobacterium sp. SH5 was a potential strain for degrading phytosterol. After mutation by UV and NTG, a mutant with 9α-hydroxylase defective was selected and named as Mycobacterium sp. SH5-52. On the basis of mutation, the nutritional and environmental conditions of Mycobacterium sp. SH5-52 were investigated and optimized. With the optimum conditions, Mycobacterium sp. SH5-52 could degrade 0.6% substance phytosterol to Androst-4-end-3,17-dione (AD) and Androst-1,4-end-3,17-dione (ADD) with the average conversion ratio significantly improved from 37.2% to 47.6%.
