CAJ | 학술논문

[目的]采用AFLP分子标记技术对北京地区鼠尾草属(Salvia spp.)植物的7个种23份样本进行亲缘关系分析.[方法]利用21对引物组合中筛选出的稳定性好、多态性较高的6对引物(E+ATT/M+AC;E+ATT/M+GA;E+AAG/M+CTG;E+AAG/M+CCT;E+AAG/M+ACT;E+AAC/M+ACT)用于扩增北京地区鼠尾草属植物基因组DNA,反应体系分为两步:①梯度降温扩增.第1个循环为94 ℃,30 s;65 ℃,30 s;72 ℃,60 s.以后每个循环的退火温度依次降低0.7 ℃,共13个循环;②普通扩增.94 ℃,30 s;56 ℃,30 s;72 ℃,60 s;23个循环.扩增后采用6%聚丙烯酰胺凝胶和BG-Power 3500型电源和DYC-20A型电泳槽,在恒功率60 W的条件下电泳至溴酚蓝指示线刚刚跑出玻璃板.银染后在灯光下统计扩增条带数,构成二进制矩阵表.用NTSYSpc 2.10e软件进行分析,采用UPGMA方法进行聚类分析,通过Tree plot模块生成聚类图.[结果]对23份鼠尾草属植物进行AFLP分析,得到367个扩增位点,其中多态性位点359个,占总扩增位点的97.82%.在遗传距离为0.32的水平下,23份鼠尾草属植物样本归为7个组:一串红(S. splendens)组、蓝花鼠尾草(S. farinacea)组、朱唇(S. coccinea)组、雪见草(S. plebeia)组、丹参(S. miltiorrhiza)组、荫生鼠尾草(S. umbratica)组和罗马尼亚鼠尾草(S.officinalis)组.蓝花鼠尾草、朱唇、雪见草、丹参、荫生鼠尾草、罗马尼亚鼠尾草与一串红的亲缘关系依次增远;一串红种内,自选品种(系)与商品种亲缘关系较远.[结论]该研究结果可为北京地区开展鼠尾草属植物的种间和种内远缘杂交提供理论参考.
[목적]채용AFLP분자표기기술대북경지구서미초속(Salvia spp.)식물적7개충23빈양본진행친연관계분석.[방법]이용21대인물조합중사선출적은정성호、다태성교고적6대인물(E+ATT/M+AC;E+ATT/M+GA;E+AAG/M+CTG;E+AAG/M+CCT;E+AAG/M+ACT;E+AAC/M+ACT)용우확증북경지구서미초속식물기인조DNA,반응체계분위량보:①제도강온확증.제1개순배위94 ℃,30 s;65 ℃,30 s;72 ℃,60 s.이후매개순배적퇴화온도의차강저0.7 ℃,공13개순배;②보통확증.94 ℃,30 s;56 ℃,30 s;72 ℃,60 s;23개순배.확증후채용6%취병희선알응효화BG-Power 3500형전원화DYC-20A형전영조,재항공솔60 W적조건하전영지추분람지시선강강포출파리판.은염후재등광하통계확증조대수,구성이진제구진표.용NTSYSpc 2.10e연건진행분석,채용UPGMA방법진행취류분석,통과Tree plot모괴생성취류도.[결과]대23빈서미초속식물진행AFLP분석,득도367개확증위점,기중다태성위점359개,점총확증위점적97.82%.재유전거리위0.32적수평하,23빈서미초속식물양본귀위7개조:일천홍(S. splendens)조、람화서미초(S. farinacea)조、주진(S. coccinea)조、설견초(S. plebeia)조、단삼(S. miltiorrhiza)조、음생서미초(S. umbratica)조화라마니아서미초(S.officinalis)조.람화서미초、주진、설견초、단삼、음생서미초、라마니아서미초여일천홍적친연관계의차증원;일천홍충내,자선품충(계)여상품충친연관계교원.[결론]해연구결과가위북경지구개전서미초속식물적충간화충내원연잡교제공이론삼고.
Twenty-third collections of Salvia spp. in Beijing were analyzed phylogenetically by AFLP technique. 6 primer pairs with good repetition and highly polymorphic bands from 21 pairs were selected to amplify the genomic DNA. All of 367 AFLP bands were obtained, in which 359 (97.82%) were polymorphic markers. At a genetic distance of 0.32, the collections could be clustered into 7 species i.e. S. splendens, S. farinacea, S. coccinea, S. plebeia, S. miltiorrhiza, S. umbratica and S. officinalis. The respective genetic relationship between S. farinacea, S. coccinea, S. plebeia, S. miltiorrhiza, S.umbratica, S. officinalis and S. splendens were further order by order. The respective genetic relationship between cultivars and commercial varieties of S. splendens was further. The results provided reference for hybrid breeding between inside and outside of species of Salvia spp. in Beijing.