中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2012年
2期
100-102
,共3页
巩本刚%徐怀勇%成丕光%高崇崇%吴俊本
鞏本剛%徐懷勇%成丕光%高崇崇%吳俊本
공본강%서부용%성비광%고숭숭%오준본
胰腺肿瘤%细胞系,肿瘤%金属蛋白酶类组织抑制剂%细胞增殖%细胞凋亡
胰腺腫瘤%細胞繫,腫瘤%金屬蛋白酶類組織抑製劑%細胞增殖%細胞凋亡
이선종류%세포계,종류%금속단백매류조직억제제%세포증식%세포조망
Pancreatic neoplasms%Cell line,tumor%Tissue inhibitor of metalloproteinases%Cell proliferation%Apoptosis
目的 探讨基质金属蛋白酶抑制剂MMI-166对人胰腺癌SW1990细胞增殖和凋亡的影响.方法 应用不同浓度(25、50、100μg/ml)的MMI-166处理人胰腺癌SW1990细胞24、48 h.用四甲基偶氮唑蓝(MTT)法检测细胞增殖抑制率；采用Annexin V-PI法检测细胞凋亡,流式细胞术检测细胞凋亡率.结果 25、50、100μg/ml MMI-166处理细胞24h后,细胞生长抑制率分别为(34.23±3.87)％、(44.81±2.01)％、(53.91±1.74)％;48 h的抑制率为(39.95±1.83)％、(52.26±3.46)％、(63.20±2.48)％,呈浓度及时间依赖性.24h的细胞凋亡率分别为(4.17±0.55)％、(8.22±0.70)％、( 14.10±0.44)％；48 h的细胞凋亡率为(11.19±0.47)％、(23.01±0.53)％、(28.10±0.52)％,均显著高于对照组的(0.09±0.12)％(P＜0.05).结论 MMI-166以浓度和时间依赖性抑制胰腺癌SW1990细胞增殖,诱导细胞凋亡.
目的 探討基質金屬蛋白酶抑製劑MMI-166對人胰腺癌SW1990細胞增殖和凋亡的影響.方法 應用不同濃度(25、50、100μg/ml)的MMI-166處理人胰腺癌SW1990細胞24、48 h.用四甲基偶氮唑藍(MTT)法檢測細胞增殖抑製率；採用Annexin V-PI法檢測細胞凋亡,流式細胞術檢測細胞凋亡率.結果 25、50、100μg/ml MMI-166處理細胞24h後,細胞生長抑製率分彆為(34.23±3.87)％、(44.81±2.01)％、(53.91±1.74)％;48 h的抑製率為(39.95±1.83)％、(52.26±3.46)％、(63.20±2.48)％,呈濃度及時間依賴性.24h的細胞凋亡率分彆為(4.17±0.55)％、(8.22±0.70)％、( 14.10±0.44)％；48 h的細胞凋亡率為(11.19±0.47)％、(23.01±0.53)％、(28.10±0.52)％,均顯著高于對照組的(0.09±0.12)％(P＜0.05).結論 MMI-166以濃度和時間依賴性抑製胰腺癌SW1990細胞增殖,誘導細胞凋亡.
목적 탐토기질금속단백매억제제MMI-166대인이선암SW1990세포증식화조망적영향.방법 응용불동농도(25、50、100μg/ml)적MMI-166처리인이선암SW1990세포24、48 h.용사갑기우담서람(MTT)법검측세포증식억제솔；채용Annexin V-PI법검측세포조망,류식세포술검측세포조망솔.결과 25、50、100μg/ml MMI-166처리세포24h후,세포생장억제솔분별위(34.23±3.87)％、(44.81±2.01)％、(53.91±1.74)％;48 h적억제솔위(39.95±1.83)％、(52.26±3.46)％、(63.20±2.48)％,정농도급시간의뢰성.24h적세포조망솔분별위(4.17±0.55)％、(8.22±0.70)％、( 14.10±0.44)％；48 h적세포조망솔위(11.19±0.47)％、(23.01±0.53)％、(28.10±0.52)％,균현저고우대조조적(0.09±0.12)％(P＜0.05).결론 MMI-166이농도화시간의뢰성억제이선암SW1990세포증식,유도세포조망.
Objective To investigate the effects of MMI-166 on the proliferation and apoptosis of human pancreatic cancer SW1990 cells.Methods MMI-166 of different concentrations (25,50,100 μg/ml) were used to treat human pancreatic cancer SW1990 cell for 24,48 h.Effect of MMI-166 on cell proliferation was detected by 3- (4,5-dimethyl-2-thiazole) -2-5-biphenly-tetrazole bromide ( MTT ) method and effect on cell apoptosis was tested by Annexin V-PI method and flow cytometry (FCM).Results Twenty-four hours after MMI-166 treatment of different concentrations (25,50,100 μg/ml),the inhibitory rates of the cells were (34.23±3.87)％,(44.81 ±2.01)％,(53.91 ±1.74)％,and the corresponding values were (39.95 ± 1.83) ％,( 52.26 ± 3.46 ) ％,( 63.20 ± 2.48 ) ％ at 48 h,which suggested a time-and concentrationdependent manner.The cell's apoptosis rates were (11.19 ±0.47)％,(23.01 ±0.53)％,(28.10 ± 0.52) ％ at 24 h,and the corresponding values were ( 11.19 ± 0.47 ) ％,( 23.01 ± 0.53 ) ％,( 28.10 ± 0.52)％ at 48 h,which were significantly higher than those in control group [ (0.09 ±0.12)％,P ＜0.05].Conclusions MMI-166 can inhibit proliferation and induce apoptosis of human pancreatic SW1990 cell in a time- and concentration-dependent manner.
