CAJ | 학술논문

目的探讨髓母细胞瘤染色体基因组DNA失衡及其与患者年龄、性别之间的关系.方法用比较基因组杂交方法对16例髓母细胞瘤的染色体基因组DNA获得和丢失进行检测.结果 16例髓母细胞瘤中,共有15例(15/16)检测到获得和(或)丢失.有获得者10例(10/16),有丢失者11例(11/16),二者的差异无统计学意义(P＞0.05);获得和丢失例数的性别及年龄差异也无统计学意义(P＞0.05).出现单染色体、双染色体、三染色体及多染色体获得和(或)丢失者分别为3例(3/15),4例(4/15),1例(1/15)和7例(7/15).该组病例共检测到11个有DNA获得(+5q、+6q、+7q、+1lq、+15q、+17p、+17q、+19q、+20q、+2lq、+Xp)和25个有DNA丢失(-1p、-1q、-2p、-2q、-3q、-4p、-6p、-6q、-8p、-8q、-10p、-10q、-11p、-14q、-16p、-16q、-17p、-18p、-18q、-19p、-19q、-20p、-20q、-Xp、-Xq)的染色体区带;以+7q(6/16)、+17q(6/16)、-14q(5/16)和-10q(3/16)最常见;且-14q均发生在＞10岁组.结论大多数髓母细胞瘤有不同程度的染色体基因组DNA失衡,常见失衡区带主要位于染色体长臂,+7q、+17q、-14q和-10q与该肿瘤的发生密切相关,-14q是导致＞10岁组髓母细胞瘤发生的重要因素,髓母细胞瘤可能存在不同的分子遗传学亚型.
목적 탐토수모세포류염색체기인조DNA실형급기여환자년령、성별지간적관계.방법 용비교기인조잡교방법대16례수모세포류적염색체기인조DNA획득화주실진행검측.결과 16례수모세포류중,공유15례(15/16)검측도획득화(혹)주실.유획득자10례(10/16),유주실자11례(11/16),이자적차이무통계학의의(P＞0.05);획득화주실례수적성별급년령차이야무통계학의의(P＞0.05).출현단염색체、쌍염색체、삼염색체급다염색체획득화(혹)주실자분별위3례(3/15),4례(4/15),1례(1/15)화7례(7/15).해조병례공검측도11개유DNA획득(+5q、+6q、+7q、+1lq、+15q、+17p、+17q、+19q、+20q、+2lq、+Xp)화25개유DNA주실(-1p、-1q、-2p、-2q、-3q、-4p、-6p、-6q、-8p、-8q、-10p、-10q、-11p、-14q、-16p、-16q、-17p、-18p、-18q、-19p、-19q、-20p、-20q、-Xp、-Xq)적염색체구대;이+7q(6/16)、+17q(6/16)、-14q(5/16)화-10q(3/16)최상견;차-14q균발생재＞10세조.결론 대다수수모세포류유불동정도적염색체기인조DNA실형,상견실형구대주요위우염색체장비,+7q、+17q、-14q화-10q여해종류적발생밀절상관,-14q시도치＞10세조수모세포류발생적중요인소,수모세포류가능존재불동적분자유전학아형.
Objective To investigate the relationship between chromosomal genomic DNA imbalance in medulloblastoma (MB), and the age and gender. Methods The gains and losses of chromosomal genomic DNA in 16 MBs were analyzed using comparative genomic hybridization. Results The gains and (or) losses were found in 15 of the 16 cases. There was not significant difference (P＞0.05)between the total gains (10/16) and losses (11/16). Both of their differences had also no significance between different age and gender groups(P＞0.05). In 15 cases with gains and (or) losses, single-, two-,three-and multi-chromosome genomic DNA imbalances were 3/15, 4/15, 1/15 and 7/15 respectively.Eleven gain zones (+5q, +6q, +7q, +11q, +1Sq, +17p, +17q, +19q, +20q, +21q, +Xp)and twenty-five loss zones(-1p,-1q, -2p, -2q, -3q, -4p, -6p, -6q, -8p, -8q, -10p,-10q, -11p, -14q, -16p, -16q, -17p, -18p, -18q,-19p, -19q, -20p, -20q,-Xp,-Xq) were detected in those tumors. +7q (6/16), + 17q (6/16), - 14q (5/16) and - 10q (3/16)were the most frequent, but - 14q only occurred in the cases of ＞ 10-year-old. Conclusions Most MBs have chromosomal genomic DNA imbalances. The frequent imbalance zones are mainly at the long arms of some chromosomes. +7q, + 17q, -14q and -10q correlate closely to development of the tumors. -14qis important factor to result in MBs of ＞ 10-year-old group. MB has possibly different molecular genetics subtype.