CAJ | 학술논문

目的探讨依达拉奉(edaravone,Ed)、神经节苷酯(GW1)对帕金森病(PD)大鼠模型的神经保护作用.方法采用立体定向一侧黑质致密部(SNC)、中脑腹侧被盖部(VTA)两点注射6-OHDA的方法 ,建立单侧PD大鼠动物模型.将老年大鼠分为正常组、生理盐水组(NS)、帕金森病模型组(PD)、PD+GM1组、PD+Ed组、PD+GM1+Ed组.14d后观察大鼠阿朴吗啡(APO)诱导的行为学改变及黑质多巴胺能神经元凋亡的情况.结果正常组、NS组APO未诱导出大鼠旋行为,SNC未见细胞凋亡.PD各组APO诱导的旋转实验＞7 r/min,旋转次数(次)PD+GM1+Ed组(8.0±0.3)＜PD+Ed组(12.0±0.6)＜PD+GM1组(17.0±1.0)＜PD组(23.0±1.3)(P＜0.01);6-OHDA可诱导细胞凋亡,毁损侧SNC细胞凋亡数(个)PD+GM1+Ed组(27.63±2.38)＜PD+Ed组(38.42±3.54)＜PD+GM1组(49.36±3.12)＜PD组(62.61±4.03)(P＜0.01).结论 6-OHDA可导致大鼠行为改变,诱导SNC神经细胞凋亡,GM1、依达拉奉降低了这种损害,依达拉奉作用强于GM1,联合治疗作用最佳.
목적 탐토의체랍봉(edaravone,Ed)、신경절감지(GW1)대파금삼병(PD)대서모형적신경보호작용.방법 채용입체정향일측흑질치밀부(SNC)、중뇌복측피개부(VTA)량점주사6-OHDA적방법 ,건립단측PD대서동물모형.장노년대서분위정상조、생리염수조(NS)、파금삼병모형조(PD)、PD+GM1조、PD+Ed조、PD+GM1+Ed조.14d후관찰대서아박마배(APO)유도적행위학개변급흑질다파알능신경원조망적정황.결과 정상조、NS조APO미유도출대서선행위,SNC미견세포조망.PD각조APO유도적선전실험＞7 r/min,선전차수(차)PD+GM1+Ed조(8.0±0.3)＜PD+Ed조(12.0±0.6)＜PD+GM1조(17.0±1.0)＜PD조(23.0±1.3)(P＜0.01);6-OHDA가유도세포조망,훼손측SNC세포조망수(개)PD+GM1+Ed조(27.63±2.38)＜PD+Ed조(38.42±3.54)＜PD+GM1조(49.36±3.12)＜PD조(62.61±4.03)(P＜0.01).결론 6-OHDA가도치대서행위개변,유도SNC신경세포조망,GM1、의체랍봉강저료저충손해,의체랍봉작용강우GM1,연합치료작용최가.
Objective To investigate the protect effects of Edaravone(Ed)and GM1 on the rat model of parkinson disease(PD).Methods To establish the unilateral PD rat model,6-OHDA was injected at two points of right substantial nigra pars compacta(SNC),ventral tegmental area(VTA),then the old rats were randomly divided into normal,NS,PD,PD + GMI,PD + Ed,FD + GM1 + Ed six groups.14d later,a rotational test induced by apomorphine was performed to determine the successful ratio.Cell apoptosis in SNC of rats were examined by TUNEL methods.Results Normal and NS groups unappeared rotate action by APO,and have no cell apoptosis in SNC.The other groups all appear rotate action(＞7 r/min)by APO,rotate action were in following gradation:PD +GM1 + Ed group(8.0±0.3)＜PD + Ed group(12.0±0.6)＜PD + GM1 group(17.0±1.0)＜PD group(23.0±1.3)(P＜0.01);and cell apoptosis in SNC were in following gradation:PD + GM1 + Ed group(27.63±2.38)＜PD + Ed group(38.42±3.54)＜PD + GM1 group(49.36±3.12)＜PD group(62.61±4.03)(P＜0.01).Conclusion 6-OHDA could induce change of action of rat and cell apoptosis in SNC.GM1,Ed reduce significantly the effect induced by 6-OHDA.GM1 combining with Ed have the best effects.