CAJ | 학술논문

目的探讨多次加热肝癌HepG2细胞后,残存细胞对化疗药耐受性与细胞凋亡变化的关系.方法 HepG2细胞43℃加热,每次80 min,每天2次,10个循环后,MTT法分析其对阿霉素的敏感性,试剂盒检测细胞的凋亡,免疫组化法检测细胞Bcl-2蛋白及Bax蛋白的表达,RT-PCR法检测细胞Bcl-2 mRNA及Bax mRNA的表达.结果热处理后的HepG2细胞对阿霉素的化疗耐受性升高,细胞凋亡率减低,Bcl-2基因/蛋白的表达增强,Bcl-2/Bax值增大.结论热处理后HepG2细胞对化疗药物耐受性增高可能与其凋亡率降低、Bcl-2基因/蛋白的表达增强及Bcl-2/Bax值增大有关.
목적 탐토다차가열간암HepG2세포후,잔존세포대화료약내수성여세포조망변화적관계.방법 HepG2세포43℃가열,매차80 min,매천2차,10개순배후,MTT법분석기대아매소적민감성,시제합검측세포적조망,면역조화법검측세포Bcl-2단백급Bax단백적표체,RT-PCR법검측세포Bcl-2 mRNA급Bax mRNA적표체.결과 열처리후적HepG2세포대아매소적화료내수성승고,세포조망솔감저,Bcl-2기인/단백적표체증강,Bcl-2/Bax치증대.결론 열처리후HepG2세포대화료약물내수성증고가능여기조망솔강저、Bcl-2기인/단백적표체증강급Bcl-2/Bax치증대유관.
Objective To investigate the change of apoptosis and drug resistance after multiple thermotherapy On the HepG2 Cell. Methods After HepG2 cell were treaded by ten repeated cycles of exposure at 43 degree C for 80 minutes twice a day，the sensibility of HepG2 cell to Adfiamyein was analyzed by MTr assay.The apoptosis Was detected by kit，and the expression of Bcl-2 or Bax protein and mRNA were detected by immunohistochemical method and RT-PCR respective.Results The drug resistance to Adfiamycin Was gained by HepG2 cell after multiple thermotherapy.The rate of apoptosis was decreased，the expression of Bcl一2 protein and mRNA Was strengthened，and the ratio of Bcl-2/Bax increased.Conclusions The resistance of heated HepG2 cell to chemotherapeutics Was related with the decreased rate of apoptosis，overexpression of Bcl-2 protein and mRNA，and increased ratio of Bcl-2/Bax.