中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2000年
1期
35-37
,共3页
周佩军%王祥慧%唐孝达%徐达%张先有%凌桂明
週珮軍%王祥慧%唐孝達%徐達%張先有%凌桂明
주패군%왕상혜%당효체%서체%장선유%릉계명
抗体,单克隆%免疫因子%基因表达
抗體,單剋隆%免疫因子%基因錶達
항체,단극륭%면역인자%기인표체
Antibodies,monoclonal%Immunologic factors%Gene expression
目的 观察CD28共刺激信号对淋巴细胞免疫活化基因表达的影响及环孢素A(CsA)对它们的抑制作用.方法 分离健康人外周血单个核细胞,分别给予抗CD3单克隆抗体(抗CD3mAb)单刺激或抗CD3 mAb+抗CD28单克隆抗体(抗CD28 mAb)共刺激培养;加或不加入CsA.于培养后6 h和24 h收集细胞.以逆转录聚合酶链反应(RT-PCR)检测β-Actin、CD40L、bcl-xL、TGF-β1、Fas、FasL、穿孔素和颗粒酶B mRNA表达水平.结果 CD28共刺激信号能显著增强免疫活化基因mRNA的表达;CsA对FasL和颗粒酶B的mRNA表达能产生抑制,而对共刺激后其余免疫活化基因的表达无明显抑制.结论 CD28共刺激信号通过提高多种T淋巴细胞相关的免疫活化基因的表达水平,而使免疫反应的活化、增殖和效应过程均得到增强;CsA对这一过程并非完全无效,仍可能具有调节作用.
目的 觀察CD28共刺激信號對淋巴細胞免疫活化基因錶達的影響及環孢素A(CsA)對它們的抑製作用.方法 分離健康人外週血單箇覈細胞,分彆給予抗CD3單剋隆抗體(抗CD3mAb)單刺激或抗CD3 mAb+抗CD28單剋隆抗體(抗CD28 mAb)共刺激培養;加或不加入CsA.于培養後6 h和24 h收集細胞.以逆轉錄聚閤酶鏈反應(RT-PCR)檢測β-Actin、CD40L、bcl-xL、TGF-β1、Fas、FasL、穿孔素和顆粒酶B mRNA錶達水平.結果 CD28共刺激信號能顯著增彊免疫活化基因mRNA的錶達;CsA對FasL和顆粒酶B的mRNA錶達能產生抑製,而對共刺激後其餘免疫活化基因的錶達無明顯抑製.結論 CD28共刺激信號通過提高多種T淋巴細胞相關的免疫活化基因的錶達水平,而使免疫反應的活化、增殖和效應過程均得到增彊;CsA對這一過程併非完全無效,仍可能具有調節作用.
목적 관찰CD28공자격신호대림파세포면역활화기인표체적영향급배포소A(CsA)대타문적억제작용.방법 분리건강인외주혈단개핵세포,분별급여항CD3단극륭항체(항CD3mAb)단자격혹항CD3 mAb+항CD28단극륭항체(항CD28 mAb)공자격배양;가혹불가입CsA.우배양후6 h화24 h수집세포.이역전록취합매련반응(RT-PCR)검측β-Actin、CD40L、bcl-xL、TGF-β1、Fas、FasL、천공소화과립매B mRNA표체수평.결과 CD28공자격신호능현저증강면역활화기인mRNA적표체;CsA대FasL화과립매B적mRNA표체능산생억제,이대공자격후기여면역활화기인적표체무명현억제.결론 CD28공자격신호통과제고다충T림파세포상관적면역활화기인적표체수평,이사면역반응적활화、증식화효응과정균득도증강;CsA대저일과정병비완전무효,잉가능구유조절작용.
Objectives To observe the effect of CD28 costimutation on lymphocyte immune activation gene expression and the suppressive effeCt of cyclosporin A(CsA).Methods Healthy peripheral blood mononuclear cells were isolated.During the culture.anti-CD3 mAb single stimulation or anti-CD3+antiCD28 mAb costimulation was given in the presence or absence of CsA.The cells were harvested 6 h and 24h after the culture.mRNA expression of β-Actin,CtNOL,bcl-xL,TGF-β1,Fas,FasL,Perforin and Granzyme B was detected by using RT-PCR technique in the samples of CD128-costimulated lymphocytes.Results CD28 costimulation could obviously enhance the expression of immune activation genes.CsA inhibited the expression of FasL and Granzyme B,but had no obvious inhibition on the expression of immune activation gene following costimulation.Conclusions CD128 costimulation could increase the expression of various lymphocyte-related immune activation gene to enhance the activation,proliferation and response of immune reaction.CsA might have modulatory effects to some extene.
