国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
5期
394-396
,共3页
中药免疫调节剂%梗阻性黄疸%幼鼠%T淋巴细胞免疫功能
中藥免疫調節劑%梗阻性黃疸%幼鼠%T淋巴細胞免疫功能
중약면역조절제%경조성황달%유서%T림파세포면역공능
Chinese herbal medicine immunomodulator%Obstructive jaundice%Immature rats%Tlymphocyte immune function
目的 探讨中药免疫调节剂对梗阻性黄疸幼鼠血液和肠黏膜T淋巴细胞免疫功能的影响.方法 以3周龄Wistar幼鼠为载体,按随机数字表法随机分为正常对照组、假手术组、梗阻性黄疸(OJ)组、OJ+中药免疫调节剂组(OJ+ZY),各12只.除正常对照组外,其余3组结扎胆总管,制作阻塞性黄疸动物模型.利用流式细胞仪检测实验幼鼠外周血及黏膜T淋巴细胞(CD4+、CD8+)的百分率,计算CD4+/CD8+比值.结果 OJ组外周血、肠黏膜CD4+T细胞[分别为(36.2±4.2)%、(28.8±1.8)%]、CD4+/CD8+比值(分别为1.14±0.39、1,37±0.34),明显低于正常对照组[外周血:CD4+(41.5±5.3)%、CD4+/CD8+(1.37±0.19);肠黏膜:CD4+(32.3±2.4)%、CD4+/CD8+(1.84±0.28)]及假手术组[外周血:CD4+(41.2±5.5)%、CD4+/CD8+(1.45±0.27);肠黏膜:CD4+(31.5±2.7)%、CD4+/CD8+(1.63±0.58)],差异均有统计学意义(P均<0.05).OJ+ZY组外周血和肠黏膜CD4+T细胞(分别为(42.7±6.3)%、(33.6±2.4)%)、CD4+/CD8+(分别为1.56±0.46、1.84±0.56)均较OJ组升高,两组比较差异有统计学意义(P均<0.05).结论 中药免疫调节剂可提高OJ幼鼠T淋巴细胞的免疫功能,而正常对照组和假手术组变化不明显.
目的 探討中藥免疫調節劑對梗阻性黃疸幼鼠血液和腸黏膜T淋巴細胞免疫功能的影響.方法 以3週齡Wistar幼鼠為載體,按隨機數字錶法隨機分為正常對照組、假手術組、梗阻性黃疸(OJ)組、OJ+中藥免疫調節劑組(OJ+ZY),各12隻.除正常對照組外,其餘3組結扎膽總管,製作阻塞性黃疸動物模型.利用流式細胞儀檢測實驗幼鼠外週血及黏膜T淋巴細胞(CD4+、CD8+)的百分率,計算CD4+/CD8+比值.結果 OJ組外週血、腸黏膜CD4+T細胞[分彆為(36.2±4.2)%、(28.8±1.8)%]、CD4+/CD8+比值(分彆為1.14±0.39、1,37±0.34),明顯低于正常對照組[外週血:CD4+(41.5±5.3)%、CD4+/CD8+(1.37±0.19);腸黏膜:CD4+(32.3±2.4)%、CD4+/CD8+(1.84±0.28)]及假手術組[外週血:CD4+(41.2±5.5)%、CD4+/CD8+(1.45±0.27);腸黏膜:CD4+(31.5±2.7)%、CD4+/CD8+(1.63±0.58)],差異均有統計學意義(P均<0.05).OJ+ZY組外週血和腸黏膜CD4+T細胞(分彆為(42.7±6.3)%、(33.6±2.4)%)、CD4+/CD8+(分彆為1.56±0.46、1.84±0.56)均較OJ組升高,兩組比較差異有統計學意義(P均<0.05).結論 中藥免疫調節劑可提高OJ幼鼠T淋巴細胞的免疫功能,而正常對照組和假手術組變化不明顯.
목적 탐토중약면역조절제대경조성황달유서혈액화장점막T림파세포면역공능적영향.방법 이3주령Wistar유서위재체,안수궤수자표법수궤분위정상대조조、가수술조、경조성황달(OJ)조、OJ+중약면역조절제조(OJ+ZY),각12지.제정상대조조외,기여3조결찰담총관,제작조새성황달동물모형.이용류식세포의검측실험유서외주혈급점막T림파세포(CD4+、CD8+)적백분솔,계산CD4+/CD8+비치.결과 OJ조외주혈、장점막CD4+T세포[분별위(36.2±4.2)%、(28.8±1.8)%]、CD4+/CD8+비치(분별위1.14±0.39、1,37±0.34),명현저우정상대조조[외주혈:CD4+(41.5±5.3)%、CD4+/CD8+(1.37±0.19);장점막:CD4+(32.3±2.4)%、CD4+/CD8+(1.84±0.28)]급가수술조[외주혈:CD4+(41.2±5.5)%、CD4+/CD8+(1.45±0.27);장점막:CD4+(31.5±2.7)%、CD4+/CD8+(1.63±0.58)],차이균유통계학의의(P균<0.05).OJ+ZY조외주혈화장점막CD4+T세포(분별위(42.7±6.3)%、(33.6±2.4)%)、CD4+/CD8+(분별위1.56±0.46、1.84±0.56)균교OJ조승고,량조비교차이유통계학의의(P균<0.05).결론 중약면역조절제가제고OJ유서T림파세포적면역공능,이정상대조조화가수술조변화불명현.
Objective To evaluate the effect of Chinese herbal medicine immunomodulator on T lymphocyte immune function in peripheral blood and intestinal mucosa of immature rats with obstructive jaundice. Methods Three-weeks Wistar rats were randomly divided into four groups. (n= 12, in each): normal control group, sham operation group, obstructive jaundice (OJ) group, OJ + Chinese herbal medicine immunomodulator (OJ+ZY) group. Except for the normal control group, the others' bile duct stones were ligatured to establish rat models with obstructive jaundice. The percentage of CD4+ and CD8+ tlymphocytes and the ratio of CD4+/CD8+ in peripheral blood and intestinal mucosa of immature rats was detected by flow cytometry. Results The percentage of CD4+ cell [(36.2 ±4.2)%, (28.8±1.8)% respectively] and the ratio of CD4+/CD8+ [(1.14±0.39), (1.37±0.34)respectively] in OJ group were lower than those in normal control group [peripheral blood: CD4+(41.5±5.3)%,CD4+/CD8+(1.37±0.19); intestinal mucosa: CD4+(32.3± 2.4)%, CD4+/CD8+ (1.84+0.28) and sham operation group (peripheral blood: CD4+ (41.2±5.5)%, CD4+/CD8+ (1.45±0.27); intestinal mucosa: CD4+(31.5 ± 2.7)%, CD4+/CD8+ (1.63±0.58)] . The difference was statistical significant(P<0.05). The percentage of CD4+ cell [(42.7±6.3)%, (33.6±2.4)% respectively] and the ratio of CD4+/CD8+ [(1.56±0.46), (1.84±0.56)respectively] in OJ+ZY group, were higher than those in OJ group(P<0.05). The difference was statistical significant(P<0.05). Conclusion Chinese herbal medicine immunomodulator can increase T lymphocyte immune function in immature rats with obstructive jaundice, but has no significance in normal control group as well as sham operation group.
