国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2012年
10期
898-900
,共3页
白附子%活性氧%人骨髓神经母细胞瘤细胞%流式细胞术
白附子%活性氧%人骨髓神經母細胞瘤細胞%流式細胞術
백부자%활성양%인골수신경모세포류세포%류식세포술
Giant typhonium rhizome%Reactive oxygen species%Human bone marrow neuroblastoma cells%Flow cytometry
目的 观察活性氧是否参与了白附子水提物抑制人骨髓神经母细胞瘤(SH-SY5Y)细胞的细胞增殖过程,以探讨白附子体外抗肿瘤作用的初步机理.方法 将白附子水提物处理人骨髓神经母细胞瘤细胞(SH-SY5Y) 24、48 h后,收集细胞,运用蛋白印记反应、流式细胞术等检测活性氧(ROS)水平及加入ROS抑制剂后的SH-SY5Y细胞凋亡水平.结果 白附子水提物诱导SH-SY5Y细胞凋亡的过程中ROS水平升高,从加药前的(4.9±1.6)％升至(39.7±11.8)％;加入ROS抑制剂后,白附子水提物诱导SH-SY5Y细胞凋亡水平降低.结论 ROS参与白附子水提物抑制SH-SY5Y的细胞增殖过程.
目的 觀察活性氧是否參與瞭白附子水提物抑製人骨髓神經母細胞瘤(SH-SY5Y)細胞的細胞增殖過程,以探討白附子體外抗腫瘤作用的初步機理.方法 將白附子水提物處理人骨髓神經母細胞瘤細胞(SH-SY5Y) 24、48 h後,收集細胞,運用蛋白印記反應、流式細胞術等檢測活性氧(ROS)水平及加入ROS抑製劑後的SH-SY5Y細胞凋亡水平.結果 白附子水提物誘導SH-SY5Y細胞凋亡的過程中ROS水平升高,從加藥前的(4.9±1.6)％升至(39.7±11.8)％;加入ROS抑製劑後,白附子水提物誘導SH-SY5Y細胞凋亡水平降低.結論 ROS參與白附子水提物抑製SH-SY5Y的細胞增殖過程.
목적 관찰활성양시부삼여료백부자수제물억제인골수신경모세포류(SH-SY5Y)세포적세포증식과정,이탐토백부자체외항종류작용적초보궤리.방법 장백부자수제물처리인골수신경모세포류세포(SH-SY5Y) 24、48 h후,수집세포,운용단백인기반응、류식세포술등검측활성양(ROS)수평급가입ROS억제제후적SH-SY5Y세포조망수평.결과 백부자수제물유도SH-SY5Y세포조망적과정중ROS수평승고,종가약전적(4.9±1.6)％승지(39.7±11.8)％;가입ROS억제제후,백부자수제물유도SH-SY5Y세포조망수평강저.결론 ROS삼여백부자수제물억제SH-SY5Y적세포증식과정.
Objective To investigate whether the reactive oxygen species involved in inhibiting proliferation of human bone marrow cells of neuroblastoma cells (SH-SY5Y)process by giant typhonium rhizome water extract,and to investigate the initial mechanism of anti-tumor effect of giant typhonium rhizome in vitro.Methods After giant typhonium rhizome water extract processing of human bone marrow neuroblastoma cells (SH-SY5Y)at 24 h and 48 h,the cells were collected,and western blotting reaction methods such as flow cytometry was used to study the levels of ROS and SH-SY5Y apoptosis.Results The levels of ROS elevated from before dosing (4.9±1.6)％ to (39.7 ± 11.8)％ in the process of giant typhonium rhizome water extract induced apoptosis in SH-SY5Y; after ROS inhibition was added,SH-SY5Y apoptosis level induced by giant typhonium rhizome water extract had decreased.Conclusion ROS involved in the process of giant typhonium rhizome water extract inhibiting cell proliferation in SH-SY5Y.
