CAJ | 학술논문

目的观察涎腺腺样囊性癌(salivary adenoid cystic carcinoma,SACC) 83细胞与鸡胚背根神经节(dorsal root ganglia,DRG)共培养模型中肿瘤细胞对神经节突起生长的诱向作用,并初步探讨发挥诱导作用的因子.方法采用玻璃底培养皿构建SACC与DRG共培养模型,SACC-83细胞接种至中央孔内,DRG组织环绕接种1周.观察神经节突起的生长情况,再分别以不同培养液对DRG培养,实验分6组:1组每孔中各加入SACC-83条件培养基、2组加入鼠成骨细胞系MC3T3-E1条件培养基、3组加入牙龈成纤维细胞条件培养基、4组加入无血清高糖达尔伯克改良伊格尔培养基(Dulbecco's modification of Eagle's medium with high glucose,H-DMEM)、5组加入含15％胎牛血清的H-DMEM、6组加入SACC-83细胞裂解液对DRG培养36 h,观察神经节突起的生长情况.结果培养36 h玻璃底培养皿共培养模型中多数神经节突起呈向中心趋向生长的状态；1组有明显的促神经节突起生长的作用,4～6组无促神经节突起生长的作用.结论以玻璃底培养皿构建肿瘤-神经共培养模型能较好地观察肿瘤细胞构成的微环境对神经突起生长的趋化作用,该作用与细胞分泌的某些特异性神经活性物质有关,该现象的存在可能与SACC嗜神经侵袭特性有关.
목적 관찰연선선양낭성암(salivary adenoid cystic carcinoma,SACC) 83세포여계배배근신경절(dorsal root ganglia,DRG)공배양모형중종류세포대신경절돌기생장적유향작용,병초보탐토발휘유도작용적인자.방법채용파리저배양명구건SACC여DRG공배양모형,SACC-83세포접충지중앙공내,DRG조직배요접충1주.관찰신경절돌기적생장정황,재분별이불동배양액대DRG배양,실험분6조:1조매공중각가입SACC-83조건배양기、2조가입서성골세포계MC3T3-E1조건배양기、3조가입아간성섬유세포조건배양기、4조가입무혈청고당체이백극개량이격이배양기(Dulbecco's modification of Eagle's medium with high glucose,H-DMEM)、5조가입함15％태우혈청적H-DMEM、6조가입SACC-83세포렬해액대DRG배양36 h,관찰신경절돌기적생장정황.결과배양36 h파리저배양명공배양모형중다수신경절돌기정향중심추향생장적상태；1조유명현적촉신경절돌기생장적작용,4～6조무촉신경절돌기생장적작용.결론이파리저배양명구건종류-신경공배양모형능교호지관찰종류세포구성적미배경대신경돌기생장적추화작용,해작용여세포분비적모사특이성신경활성물질유관,해현상적존재가능여SACC기신경침습특성유관.
Objective To construct the co-culture models of salivarya denoid cystic carcinoma (SACC) cells and dorsal root ganglia (DRG) of chickens and investigate the promotive effects of SACC on neural tissue.Methods Glass-base culture dish was adopted to construct co-culture model of SACC-83 cells and DRG. SACC-83 cells were seeded in the medium pore with DRG around them. Outgrowth of neuronal processes was observed.Then DRG was cultured in the conditioned medium of SACC-83,with the groups of conditioned medium of MC3T3-E1 and HGF,the group of cell lysis buffer,the groups of serum free medium and serum-plus medium as the controls.Outgrowth of neuronal processes was also recorded and compared with control groups.Results In the co-culture model of tumor and neuronal tissue,SACC-83cells produced a suitable microenvironment in which neuronal processes remarkably grow. Neuronal processes of most DRG displayed growth tendency toward SACC.The group of conditioned medium from SACC-83 manifested obvious promotive effects on DRG. Conclusions Co-culture model of tumor and neuronal tissue was successfully constructed,with which the promotive effects of tumor on outgrowth of neuronal processes could be observed.So hypothesized that SACC could secrete some neurotrophic factors to guide peripheral nerves gemmating and to trigger the cascade of the neural invasion in succession.